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PMID: 2459710 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Localized maternal mRNA related to transforming growth factor beta mRNA is concentrated in a cytokeratin-enriched fraction from Xenopus oocytes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 20 ·1988-10-00 ·Pages 7612-6

Pondel MD, King ML

Abstract

The localized maternal RNA Vg1 resides in the cortical region of the vegetal pole of fully grown Xenopus oocytes and is inherited by only a subset of blastomeres in the early embryo [Weeks, D. L. & Melton, D. A. (1987) Cell 51, 861-867]. Because RNA-cytoskeletal interactions may play a role in RNA localization, we have examined the association of Vg1 RNA with components of the oocyte's cytoskeleton. Gel and immunoblot analysis of a detergent-insoluble fraction revealed a greatly simplified protein pattern composed largely of cytokeratins and vimentin. In sharp contrast to the nonlocalized histone H3 mRNA, Vg1 RNA was concentrated some 35- to 50-fold in this insoluble fraction. Extractions at higher salt concentrations yielded preparations further enriched in cytokeratins and in the Vg1 RNA. Upon ovulation, VG1 RNA is released into the soluble fraction. This change in Vg1 RNA distribution coincides with the observed breakdown of cortical cytokeratin filaments [Klymkowsky, M. W., Maynell, L. A. & Polson, A. G. (1987) Development 100, 543-557] and the loss of Vg1 RNA from the cortical region. Our findings are consistent with the hypothesis that RNA-cytoskeletal interactions are involved in the localization and segregation of information during development.

MeSH Terms
Animals Autoradiography Blotting, Northern Cross Reactions Cytoskeleton/analysis Electrophoresis, Polyacrylamide Gel Female Immunoblotting Keratins/analysis Nucleic Acid Hybridization Oocytes/analysis,ultrastructure Poly A/analysis RNA, Messenger/analysis Transforming Growth Factors/genetics Vimentin/analysis Xenopus laevis
Chemicals
RNA, Messenger Vimentin Poly A Keratins Transforming Growth Factors
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Pondel M D
Department of Anatomy and Cell Biology, University of Virginia School of Medicine, Charlottesville 22908.
King M L
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.20.7612
Published
1988-10-00
Pages
7612-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC282242
Subset
IM
Grants
NIGMS NIH HHS · GM33392 · United States
NICHD NIH HHS · HD07192 · United States
External Links
PubMed source DOI: 10.1073/pnas.85.20.7612 PMC Full Text View journal details
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