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PMID: 2459131 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Evidence that an RGD-dependent receptor mediates the binding of oligodendrocytes to a novel ligand in a glial-derived matrix.

The Journal of cell biology ·Vol. 107 ·No. 4 ·1988-10-00 ·Pages 1541-9

Cardwell MC, Rome LH

Abstract

A simple adhesion assay was used to measure the interaction between rat oligodendrocytes and various substrata, including a matrix secreted by glial cells. Oligodendrocytes bound to surfaces coated with fibronectin, vitronectin and a protein component of the glial matrix. The binding of cells to all of these substrates was inhibited by a synthetic peptide (GRGDSP) modeled after the cell-binding domain of fibronectin. The component of the glial matrix responsible for the oligodendrocyte interaction is a protein which is either secreted by the glial cells or removed from serum by products of these cultures; serum alone does not promote adhesion to the same extent as the glial-derived matrix. The interaction of cells with this glial-derived matrix requires divalent cations and is not mediated by several known RGD-containing extracellular proteins, including fibronectin, vitronectin, thrombospondin, type I and type IV collagen, and tenascin.

MeSH Terms
Animals Binding, Competitive Blotting, Western Cell Adhesion Cells, Cultured Edetic Acid/pharmacology Extracellular Matrix/physiology Fibronectins/metabolism Glycoproteins/metabolism Ligands Neuroglia/cytology Oligodendroglia/cytology Oligopeptides/metabolism Rats Receptors, Cell Surface/physiology Receptors, Immunologic/physiology Receptors, Peptide Vitronectin
Chemicals
Fibronectins Glycoproteins Ligands Oligopeptides Receptors, Cell Surface Receptors, Immunologic Receptors, Peptide Vitronectin arginyl-glycyl-aspartic acid directed cell adhesion receptor Edetic Acid
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Cardwell M C
Department of Biological Chemistry, UCLA School of Medicine 90024.
Rome L H
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49 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.107.4.1541
Published
1988-10-00
Pages
1541-9
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2115253
Subset
IM
Grants
NICHD NIH HHS · HD-06576 · United States
NICHD NIH HHS · HD-07032 · United States
External Links
PubMed source DOI: 10.1083/jcb.107.4.1541 PMC Full Text View journal details
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