Immunobiology of gonococcal outer membrane protein I.

Antonie van Leeuwenhoek ·Vol. 53 ·No. 6 ·1987-00-00 ·Pages 461-4

Heckels JE, Virji M, Zak K, Fletcher JN

Abstract

Monoclonal antibodies have been obtained which react with gonococcal outer membrane protein I. One antibody recognised the majority of strains expressing P.IA and another recognised the majority of strains expressing P.IB. In in vitro tests both antibodies were bactericidal in the presence of complement, opsonic for phagocytosis by human PMN and protected epithelial cells against gonococcal invasion. Thus conserved epitopes on P.I. are potentially effective targets for immunoprophylaxis.

MeSH Terms

Animals Antibodies, Monoclonal/biosynthesis,immunology Antigens, Bacterial/immunology Antigens, Surface/immunology Bacterial Outer Membrane Proteins/immunology Binding, Competitive Epitopes/analysis,immunology Hybridomas Mice Neisseria gonorrhoeae/immunology Porins Radioimmunoassay

Chemicals

Antibodies, Monoclonal Antigens, Bacterial Antigens, Surface Bacterial Outer Membrane Proteins Epitopes Porins porin protein, Neisseria

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Heckels J E

Dept. of Microbiology, University of Southampton, Medical School, Southampton General Hospital, UK.

Virji M

Zak K

Fletcher J N

References (5)

5 references, click to expand

Role of anti-pilus antibodies in host defense against gonococcal infection studied with monoclonal anti-pilus antibodies.
Infect Immun. 1985 Sep;49(3):621-8 PMID: 2411660
The effect of protein II and pili on the interaction of Neisseria gonorrhoeae with human polymorphonuclear leucocytes.
J Gen Microbiol. 1986 Feb;132(2):503-12 PMID: 2872268
Monoclonal antibodies to gonococcal outer membrane protein IB: use in investigation of the potential protective effect of antibodies directed against conserved and type-specific epitopes.
J Gen Microbiol. 1986 Jun;132(6):1621-9 PMID: 2433385
Monoclonal antibodies to gonococcal outer membrane protein I: location of a conserved epitope on protein IB.
J Gen Microbiol. 1986 Jun;132(6):1611-20 PMID: 2433384
Serology of Neisseria gonorrhoeae: coagglutination serogroups WI and WII/III correspond to different outer membrane protein I molecules.
Infect Immun. 1982 Nov;38(2):462-70 PMID: 6183215

Article Info

Journal

Antonie van Leeuwenhoek

Abbr.

Antonie Van Leeuwenhoek

ISSN

0003-6072

DOI

10.1007/BF00415503

Published

1987-00-00

Pages

461-4

Language

English

Region

Netherlands

NLM ID

0372625

Subset

External Links

PubMed source DOI: 10.1007/BF00415503 View journal details

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Immunobiology of gonococcal outer membrane protein I.

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