Home LiteratureArticle Details
PMID: 2448310 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Nuclear transport in 3T3 fibroblasts: effects of growth factors, transformation, and cell shape.

The Journal of cell biology ·Vol. 106 ·No. 1 ·1988-01-00 ·Pages 13-9

Jiang LW, Schindler M

Abstract

Nucleocytoplasmic transport of fluorescent-labeled macromolecules was investigated in transformed and nontransformed 3T3 fibroblasts. Insulin and epidermal growth factor enhanced transport three-fold after 1-2-h incubation with nontransformed adhering fibroblasts; no enhancement of transport was observed for spherical unattached fibroblasts. The concentration of growth factor for maximal enhancement was 3-10 nM. Nuclear transport for Kirsten murine sarcoma virus-transformed BALB/c 3T3 fibroblasts, however, was maximally enhanced before addition of growth factors; addition of insulin or epidermal growth factor causes no additional transport enhancement. Transformation also minimizes cell shape effects on macromolecular nuclear transport. These results provide evidence that protein growth factors and oncogenic transformation may use a similar mechanism for activation of nuclear transport.

MeSH Terms
Animals Biological Transport Cell Line Cell Nucleus/metabolism Cell Transformation, Neoplastic/metabolism Cell Transformation, Viral Cytoplasm/metabolism Dextrans/metabolism Diffusion Epidermal Growth Factor/pharmacology Growth Substances/pharmacology Insulin/pharmacology Mice Nuclear Envelope/metabolism Receptors, Cell Surface/physiology Spectrometry, Fluorescence
Chemicals
Dextrans Growth Substances Insulin Receptors, Cell Surface Epidermal Growth Factor
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Jiang L W
Department of Biochemistry, Michigan State University, East Lansing 48824.
Schindler M
References (35)
35 references, click to expand
  1. Autocrine growth factors and cancer.
    Nature. 1985 Feb 28-Mar 6;313(6005):745-7 PMID: 3883191
  2. Epidermal growth factor and insulin stimulate nuclear pore-mediated macromolecular transport in isolated rat liver nuclei.
    J Cell Biol. 1987 Apr;104(4):849-53 PMID: 2435740
  3. Insulin regulation of protein phosphorylation in isolated rat liver nuclear envelopes: potential relationship to mRNA metabolism.
    Proc Natl Acad Sci U S A. 1983 Mar;80(5):1189-93 PMID: 6298783
  4. Chromatin binding of epidermal growth factor, nerve growth factor, and platelet-derived growth factor in cells bearing the appropriate surface receptors.
    Proc Natl Acad Sci U S A. 1986 Jun;83(11):3728-32 PMID: 3012531
  5. Early signals in the mitogenic response.
    Science. 1986 Oct 10;234(4773):161-6 PMID: 3018928
  6. Nerve growth factor in the nucleus: interaction with receptors on the nuclear membrane.
    Proc Natl Acad Sci U S A. 1979 Mar;76(3):1269-73 PMID: 286309
  7. Insulin binding to isolated liver nuclei from obese and lean mice.
    Biochemistry. 1979 Aug 21;18(17):3674-9 PMID: 476079
  8. Molecular mobility and nucleocytoplasmic flux in hepatoma cells.
    J Cell Biol. 1986 Apr;102(4):1183-90 PMID: 2420804
  9. Purification and characterization of epidermal growth factor receptor/protein kinase from normal mouse liver.
    Proc Natl Acad Sci U S A. 1982 Oct;79(20):6237-41 PMID: 6983068
  10. Epidermal growth factor-nonresponsive 3T3 variants do not contain epidermal growth factor receptor-related antigens or mRNA.
    Proc Natl Acad Sci U S A. 1986 Jan;83(2):333-6 PMID: 3001732
  11. The fate of insulin bound to adipocytes. Evidence for compartmentalization and processing.
    J Biol Chem. 1978 Jul 25;253(14):4900-6 PMID: 670168
  12. Fluorescence photobleaching assay of tumor promoter 12-O-tetradecanoylphorbol 13-acetate inhibition of cell-cell communication.
    Methods Enzymol. 1987;141:439-47 PMID: 3600363
  13. Epidermal growth factor stimulates phosphorylation in membrane preparations in vitro.
    Nature. 1978 Nov 23;276(5686):409-10 PMID: 309559
  14. Sequence requirements for nuclear location of simian virus 40 large-T antigen.
    Nature. 1984 Sep 6-11;311(5981):33-8 PMID: 6088992
  15. Ultrastructural evidence for the accumulation of insulin in nuclei of intact 3T3-L1 adipocytes by an insulin-receptor mediated process.
    Proc Natl Acad Sci U S A. 1987 Jan;84(2):459-63 PMID: 3540967
  16. The biology and mechanism of action of nerve growth factor.
    Annu Rev Biochem. 1982;51:845-68 PMID: 6287924
  17. 125I-labeled human epidermal growth factor. Binding, internalization, and degradation in human fibroblasts.
    J Cell Biol. 1976 Oct;71(1):159-71 PMID: 977646
  18. A method for incorporating macromolecules into adherent cells.
    J Cell Biol. 1984 Apr;98(4):1556-64 PMID: 6201494
  19. A fluorescence photobleaching assay of gap junction-mediated communication between human cells.
    Science. 1986 Apr 25;232(4749):525-8 PMID: 3961495
  20. Intracellular distribution of nerve growth factor in rat pheochromocytoma PC12 cells: evidence for a perinuclear and intranuclear location.
    Proc Natl Acad Sci U S A. 1980 Mar;77(3):1656-60 PMID: 6990413
  21. Fluorescence redistribution after photobleaching as a tool for dissecting the control elements of nucleocytoplasmic transport.
    Methods Enzymol. 1987;141:447-59 PMID: 2439876
  22. Perinuclear location and recycling of epidermal growth factor receptor kinase: immunofluorescent visualization using antibodies directed to kinase and extracellular domains.
    J Cell Biol. 1986 Aug;103(2):333-42 PMID: 2426278
  23. Chemical factors that influence nucleocytoplasmic transport: a fluorescence photobleaching study.
    J Cell Biol. 1986 Mar;102(3):853-8 PMID: 2419344
  24. Nuclear accumulation of epidermal growth factor in cultured rat pituitary cells.
    Nature. 1980 Sep 25;287(5780):340-3 PMID: 6968406
  25. Variants of 3T3 cells lacking mitogenic response to epidermal growth factor.
    Proc Natl Acad Sci U S A. 1977 Sep;74(9):3918-21 PMID: 302945
  26. Protein-tyrosine kinases.
    Annu Rev Biochem. 1985;54:897-930 PMID: 2992362
  27. Biosynthesis and mechanism of action of nerve growth factor.
    Recent Prog Horm Res. 1981;37:417-46 PMID: 6269164
  28. The nuclear envelope. The major site of insulin binding in rat liver nuclei.
    J Biol Chem. 1978 Apr 10;253(7):2098-103 PMID: 24634
  29. Visualization by fluorescence of the binding and internalization of epidermal growth factor in human carcinoma cells A-431.
    Proc Natl Acad Sci U S A. 1978 Jul;75(7):3317-21 PMID: 356052
  30. Role of cell shape in growth control.
    Nature. 1978 Jun 1;273(5661):345-9 PMID: 661946
  31. Nuclear accumulation of epidermal growth factor in cultured bovine corneal endothelial and granulosa cells.
    J Biol Chem. 1981 Feb 10;256(3):1149-54 PMID: 6969726
  32. Insulin-modulated transport of RNA from isolated live nuclei.
    Arch Biochem Biophys. 1981 Aug;210(1):275-9 PMID: 6170261
  33. Polypeptide growth factors.
    Annu Rev Biochem. 1984;53:259-92 PMID: 6089648
  34. Binding of insulin to isolated nuclei.
    Proc Natl Acad Sci U S A. 1976 May;73(5):1427-31 PMID: 179086
  35. Receptors and phosphoinositide-generated second messengers.
    Annu Rev Biochem. 1985;54:205-35 PMID: 2992357
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.106.1.13
Published
1988-01-00
Pages
13-9
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2114954
Subset
IM
Grants
NIGMS NIH HHS · GM 30158 · United States
External Links
PubMed source DOI: 10.1083/jcb.106.1.13 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2