Fractionation of the enzymes of the barley aleurone layer: Evidence for a soluble mode of enzyme release.

Planta ·Vol. 103 ·No. 2 ·1972-06-00 ·Pages 95-109

Jones RL

Abstract

Aleurone cells of barley (Hordeum vulgare L.) contain microbodies as determined by histochemical localization with diaminobenzidine. These microbodies can be isolated from both water and gibberellic acid (GA3) treated cells and identified on sucrose density gradients as glyoxysomes on the basis of their buoyant densities (1.25 g cm(-3)) and their enzyme complement. Fractionation of aleurone layer homogenates by differential centrifugation after varying times of exposure to GA3, however, does not indicate the presence of a discrete secretory vesicle containing either α-amylase or β-1,3-glucanase. Cytological evidence also suggests that at least β-1,3-glucanase is not released from these cells by means of a discrete secretory vesicle.

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Jones R L

Department of Botany, University of California, Berkeley, California, U.S.A..

References (20)

20 references, click to expand

Gibberellic Acid-induced synthesis of protease by isolated aleurone layers of barley.
Plant Physiol. 1967 Nov;42(11):1596-600 PMID: 16656695
Gibberellic acid and the fine structure of barley aleurone cells : II. Changes during the synthesis and secretion of α-amylase.
Planta. 1969 Mar;88(1):73-86 PMID: 24504839
Role of the Golgi complex in the intracellular transport of secretory proteins.
Proc Natl Acad Sci U S A. 1966 Feb;55(2):424-31 PMID: 5220960
Gibberellic acid and the fine structure of barley aleurone cells : III. Vacuolation of the Aleurone cell during the phase of ribonuclease release.
Planta. 1970 Sep;94(3):191-202 PMID: 24496935
Visualization of peroxisomes (microbodies) and mitochondria with diaminobenzidine.
J Histochem Cytochem. 1969 Oct;17(10):675-80 PMID: 4194356
Gibberellic acid and the fine structure of barley aleurone cells : I. Changes during the lag-phase of α-amylase synthesis.
Planta. 1969 Mar;87(1-2):119-33 PMID: 24504722
Gibberellic acid, β-1,3-glucanase and the cell walls of barley aleurone layers.
Planta. 1970 Mar;92(1):73-84 PMID: 24500132
Association of the glyoxylate cycle enzymes in a novel subcellular particle from castor bean endosperm.
Biochem Biophys Res Commun. 1967 May 25;27(4):462-9 PMID: 6052476
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
Intracellular transport of secretory proteins in the pancreatic exocrine cell. II. Transport to condensing vacuoles and zymogen granules.
J Cell Biol. 1967 Aug;34(2):597-615 PMID: 6035648
ISOLATION OF beta-GLUCAN SYNTHETASE PARTICLES FROM PLANT CELLS AND IDENTIFICATION WITH GOLGI MEMBRANES.
Proc Natl Acad Sci U S A. 1969 Oct;64(2):605-12 PMID: 16591795
A test for de novo synthesis of enzymes: density labeling with H2O18 of barley alpha-amylase induced by gibberellic acid.
Proc Natl Acad Sci U S A. 1967 Oct;58(4):1520-6 PMID: 5237885
Isolation of glucanase-containing particles from budding Saccharomyces cerevisiae.
Proc Natl Acad Sci U S A. 1971 Mar;68(3):636-40 PMID: 5276773
Structure of isolated plant Golgi apparatus revealed by negative staining.
J Cell Biol. 1966 Feb;28(2):169-79 PMID: 4161888
Ultrastructure and distribution of microbodies in leaves of grasses with and without CO2-photorespiration.
Planta. 1971 Jun;96(2):152-74 PMID: 24493087
Developmental studies on glyoxysomes in Ricinus endosperm.
J Cell Biol. 1970 Jan;44(1):94-102 PMID: 5409466
Intracellular transport of secretory proteins in the pancreatic exocrine cell. I. Role of the peripheral elements of the Golgi complex.
J Cell Biol. 1967 Aug;34(2):577-96 PMID: 6035647
Gibberellic Acid-enhanced Release of beta-1,3-Glucanase from Barley Aleurone Cells.
Plant Physiol. 1971 Mar;47(3):412-6 PMID: 16657633
Characterization of glyoxysomes from castor bean endosperm.
Plant Physiol. 1968 May;43(5):705-13 PMID: 16656830
Gibberellic Acid-enhanced synthesis and release of alpha-amylase and ribonuclease by isolated barley and aleurone layers.
Plant Physiol. 1967 Mar;42(3):398-406 PMID: 16656517

Article Info

Journal

Planta

Abbr.

Planta

ISSN

0032-0935

DOI

10.1007/BF00387360

Published

1972-06-00

Pages

95-109

Language

English

Region

Germany

NLM ID

1250576

External Links

PubMed source DOI: 10.1007/BF00387360 View journal details

Analysis Services

Fractionation of the enzymes of the barley aleurone layer: Evidence for a soluble mode of enzyme release.

Contact

WeChat Official Account