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题目:: Perinucleolar relocalization and nucleolin as crucial events in the transcriptional activation of key genes in mantle cell lymphoma.
作者:: Allinne(Jeanne),Pichugin(Andrei),Iarovaia(Olga),Klibi(Manel),Barat(Ana),Zlotek-Zlotkiewicz(Ewa),Markozashvili(Diana),Petrova(Natalia),Camara-Clayette(Valérie),Ioudinkova(Elena),Wiels(Joëlle),Razin(Sergey V),Ribrag(Vincent),Lipinski(Marc),Vassetzky(Yegor S)
状态:: 发布时间2014-03-28 , 更新时间 2014-03-28
期刊:: Blood
摘要:: In mantle cell lymphoma (MCL), one allele of the cyclin D1 (Ccnd1) gene is translocated from its normal localization on chromosome 11 to chromosome 14. This is considered as the crucial event in the transformation process of a normal naive B-cell; however, the actual molecular mechanism leading to Ccnd1 activation remains to be deciphered. Using a combination of three-dimensional and immuno-fluorescence in situ hybridization experiments, the radial position of the 2 Ccnd1 alleles was investigated in MCL-derived cell lines and malignant cells from affected patients. The translocated Ccnd1 allele was observed significantly more distant from the nuclear membrane than its nontranslocated counterpart, with a very high proportion of IgH-Ccnd1 chromosomal segments localized next to a nucleolus. These perinucleolar areas were found to contain active RNA polymerase II (PolII) clusters. Nucleoli are rich in nucleolin, a potent transcription factor that we found to bind sites within the Ccnd1 gene specifically in MCL cells and to activate Ccnd1 transcription. We propose that the Ccnd1 transcriptional activation in MCL cells relates to the repositioning of the rearranged IgH-Ccnd1-carrying chromosomal segment in a nuclear territory with abundant nucleolin and active PolII molecules. Similar transforming events could occur in Burkitt and other B-cell lymphomas.
语言:: eng
DOI:: 10.1182/blood-2013-06-510511

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