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PMID: 2444601 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Electron microscopic observations of reconstituted proteoliposomes with the purified major intrinsic membrane protein of eye lens fibers.

The Journal of cell biology ·Vol. 105 ·No. 4 ·1987-10-00 ·Pages 1679-89

Dunia I, Manenti S, Rousselet A, Benedetti EL

Abstract

The purified major intrinsic protein of the lens fiber plasma membrane (MP26) reconstituted into liposomes favored membrane-to-membrane close contacts as visualized by freeze fracture and immunoelectron microscopy. Reconstituted apposed unilamellar vesicles formed pentalaminar profiles, and multilamellar liposomes showed regions of stacked bilayers. Immunogold labeling, using antibody directed against MP26, demonstrated that this polypeptide is present in regions of membrane-to-membrane close interaction. Fracture faces displayed both randomly distributed clusters of 8-nm polygonal intramembrane particles and membrane domains where a bidimensional lattice of repeating subunits was present. The structural pleomorphism which characterized the MP26-reconstituted proteoliposomes seems quite comparable to that visualized in natural fiber plasma membrane domains.

MeSH Terms
Animals Aquaporins Cattle Eye Proteins/physiology Freeze Fracturing Immunohistochemistry Immunosorbent Techniques Intercellular Junctions/ultrastructure Ion Channels Lens, Crystalline/ultrastructure Liposomes Membrane Glycoproteins Membrane Proteins/physiology Microscopy, Electron Molecular Weight
Chemicals
Aquaporins Eye Proteins Ion Channels Liposomes Membrane Glycoproteins Membrane Proteins aquaporin 0
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Dunia I
Institut Jacques Monod--Centre National de la Recherche Scientifique CNRS, Université Paris VII, France.
Manenti S
Rousselet A
Benedetti E L
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.105.4.1679
Published
1987-10-00
Pages
1679-89
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2114674
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.105.4.1679 PMC Full Text View journal details
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