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PMID: 2444534 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Antigenic determinants shared between HLA-A, -B, -C antigens and H-2 class I molecules modified by bovine beta-2 microglobulin.

Immunogenetics ·Vol. 26 ·No. 6 ·1987-00-00 ·Pages 351-5

Mierau R, Robinson PJ, Sanderson AR, Genth E, Cramer M

Abstract

The specificity of the mouse class I-specific antibody COB6-3 was examined in detail. It was found to react with the mouse class I molecules H-2Db, Kd, and Qa-2, and with human HLA-A, -B, -C antigens. The specificity pattern of COB6-3, despite its different origin, was similar to that of the monomorphic HLA class I-specific antibody W6/32. Cross-inhibition studies show that on human cells the antigenic determinants recognized by the two antibodies are situated close together and may be identical. On mouse cells, reactivity of both antibodies was generated upon replacement of mouse beta-2 microglobulin (B2m) with its bovine counterpart, but differences in specificity were observed using human B2m.

MeSH Terms
Animals Antibodies, Monoclonal/immunology Antibody Specificity Binding Sites, Antibody Cattle Cell Line Cross Reactions Epitopes/genetics,immunology H-2 Antigens/genetics,immunology HLA Antigens/genetics,immunology HLA-A Antigens HLA-B Antigens HLA-C Antigens Humans Mice Mice, Inbred C57BL beta 2-Microglobulin/immunology
Chemicals
Antibodies, Monoclonal Epitopes H-2 Antigens HLA Antigens HLA-A Antigens HLA-B Antigens HLA-C Antigens beta 2-Microglobulin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Mierau R
Rheumaforschungsinstitut, Rheumaklinik Aachen, Federal Republic of Germany.
Robinson P J
Sanderson A R
Genth E
Cramer M
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Article Info
Journal
Immunogenetics
Abbr.
Immunogenetics
ISSN
0093-7711
DOI
10.1007/BF00343703
Published
1987-00-00
Pages
351-5
Language
English
Region
United States
NLM ID
0420404
Subset
IM
Corrections
ErratumIn
-
External Links
PubMed source DOI: 10.1007/BF00343703 View journal details
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