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PMID: 2444213 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Rapid subcellular fractionation of the rat liver endocytic compartments involved in transcytosis of polymeric immunoglobulin A and endocytosis of asialofetuin.

The Biochemical journal ·Vol. 244 ·No. 2 ·1987-06-01 ·Pages 311-5

Branch WJ, Mullock BM, Luzio JP

Abstract

The distributions of two endocytosed radiolabelled ligands (polymeric immunoglobulin A and asialofetuin) in rat liver endocytic compartments were investigated by using rapid subcellular fractionation of post-mitochondrial supernatants on vertical density gradients of Ficoll or Nycodenz. Two endocytic compartments were identified, both ligands being initially associated with a light endocytic-vesicle fraction on Ficoll gradients, asialofetuin then accumulating in denser endosomes before transfer to lysosomes for degradation.

MeSH Terms
Animals Asialoglycoproteins Cell Compartmentation Centrifugation, Isopycnic Endocytosis Fetuins Immunoglobulin A/metabolism Liver/metabolism Male Rats Rats, Inbred Strains Subcellular Fractions/metabolism alpha-Fetoproteins/metabolism
Chemicals
Asialoglycoproteins Fetuins Immunoglobulin A alpha-Fetoproteins asialofetuin polymeric IgA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Branch W J
Department of Clinical Biochemistry, University of Cambridge, Addenbrooke's Hospital, U.K.
Mullock B M
Luzio J P
References (17)
17 references, click to expand
  1. Uptake and degradation of 125I-labelled asialo-fetuin by isolated rat hepatocytes.
    Biochim Biophys Acta. 1977 Aug 25;499(1):73-84 PMID: 70230
  2. Subfractionation of hepatic endosomes in Nycodenz gradients and by free-flow electrophoresis. Separation of ligand-transporting and receptor-enriched membranes.
    Biochem J. 1985 Nov 15;232(1):25-32 PMID: 2867760
  3. Inhibition of 125I-asialofetuin catabolism by leupeptin in the perfused rat liver and in vivo.
    J Biol Chem. 1979 May 25;254(10):4191-6 PMID: 86540
  4. The effects of colchicine and cytochalasin B on uptake and degradation of asialo-glycoproteins in isolated rat hepatocytes.
    Exp Cell Res. 1979 Aug;122(1):159-67 PMID: 477753
  5. Subcellular distribution and movement of 5'-nucleotidase in rat cells.
    Biochem J. 1980 Jan 15;186(1):59-69 PMID: 6245642
  6. Effect of colchicine on the transfer of IgA across hepatocytes into bile in isolated perfused rat livers.
    FEBS Lett. 1980 Nov 3;120(2):278-82 PMID: 7439402
  7. Kinetics of internalization and degradation of asialo-glycoproteins in isolated rat hepatocytes.
    Eur J Cell Biol. 1980 Dec;23(1):104-9 PMID: 6161816
  8. Preparation of a low-density species of endocytic vesicle containing immunoglobulin A.
    Biochem J. 1983 Sep 15;214(3):823-7 PMID: 6626159
  9. The isolation of endosome-derived vesicles from rat hepatocytes.
    Biochem J. 1983 Oct 15;216(1):27-36 PMID: 6316938
  10. Receptor-mediated biliary transport of immunoglobulin A and asialoglycoprotein: sorting and missorting of ligands revealed by two radiolabeling methods.
    J Cell Biol. 1984 Jan;98(1):79-89 PMID: 6323485
  11. Intracellular receptor sorting during endocytosis: comparative immunoelectron microscopy of multiple receptors in rat liver.
    Cell. 1984 May;37(1):195-204 PMID: 6327050
  12. Two populations of prelysosomal structures transporting asialoglycoproteins in rat liver.
    Proc Natl Acad Sci U S A. 1984 May;81(10):2995-9 PMID: 6587338
  13. Receptor-mediated endocytosis of polymeric IgA and galactosylated serum albumin in rat liver. Evidence for intracellular ligand sorting and identification of distinct endosomal compartments.
    Eur J Biochem. 1985 Feb 1;146(3):539-48 PMID: 2982599
  14. Separation of endocytic vesicles in Nycodenz gradients.
    Anal Biochem. 1984 Nov 1;142(2):455-62 PMID: 6084961
  15. Intracellular transport of asialoglycoproteins in rat hepatocytes. Evidence for two subpopulations of lysosomes.
    Exp Cell Res. 1985 Dec;161(2):285-96 PMID: 4065221
  16. Transcytosis.
    Cell. 1985 Dec;43(2 Pt 1):389-90 PMID: 4075399
  17. Rapid active transport of immunoglobulin A from blood to bile.
    J Exp Med. 1978 Feb 1;147(2):588-92 PMID: 624907
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2440311
Published
1987-06-01
Pages
311-5
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1147992
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2440311 PMC Full Text View journal details
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