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PMID: 24407390 Published · ppublish English Journal Article

Cell free synthesis of some storage protein subunits by polyribosomes and RNA isolated from developing seeds of pea (Pisum sativum L.).

Planta ·Vol. 144 ·No. 5 ·1979-01-00 ·Pages 455-62

Marta Evans I, Croy RR, Hutchinson P, Boulter D, Payne PI, Gordon ME

Abstract

Polyribosomes which have template activity in the wheat germ system have been isolated from developing pea seeds. Some of the translation products have identical mobilities to the vicilin and legumin subunits by SDS-PAGE. Certain products were specifically immunoprecipitated with antisera prepared against purified vicilin and legumin fractions. Various RNA fractions including poly A-rich RNA have also been isolated from polyribosomes and shown to direct the synthesis of polyripeptides whose properties are similar to the storage protein subunits. The results are discussed in relationship to other investigations with seed storage protein biosynthesis in vitro.

Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Marta Evans I
Department of Botany, University of Durham, DH1 3LE, Durham, U.K..
Croy R R
Hutchinson P
Boulter D
Payne P I
Gordon M E
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Article Info
Journal
Planta
Abbr.
Planta
ISSN
0032-0935
DOI
10.1007/BF00380122
Published
1979-01-00
Pages
455-62
Language
English
Region
Germany
NLM ID
1250576
External Links
PubMed source DOI: 10.1007/BF00380122 View journal details
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