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PMID: 24406630 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

CO2 transport by PIP2 aquaporins of barley.

Plant & cell physiology ·Vol. 55 ·No. 2 ·2014-02-00 ·Pages 251-7

Mori IC, Rhee J, Shibasaka M, Sasano S, Kaneko T, Horie T, Katsuhara M

Abstract

CO2 permeability of plasma membrane intrinsic protein 2 (PIP2) aquaporins of Hordeum vulgare L. was investigated. Five PIP2 members were heterologously expressed in Xenopus laevis oocytes. CO2 permeability was determined by decrease of cytosolic pH in CO2-enriched buffer using a hydrogen ion-selective microelectrode. HvPIP2;1, HvPIP2;2, HvPIP2;3 and HvPIP2;5 facilitated CO2 transport across the oocyte cell membrane. However, HvPIP2;4 that is highly homologous to HvPIP2;3 did not. The isoleucine residue at position 254 of HvPIP2;3 was conserved in PIP2 aquaporins of barley, except HvPIP2;4, which possesses methionine instead. CO2 permeability was lost by the substitution of the Ile254 of HvPIP2;3 by methionine, while water permeability was not affected. These results suggest that PIP2 aquaporins are permeable to CO2. and the conserved isoleucine at the end of the E-loop is crucial for CO2 selectivity.

Keywords
Aquaporin Barley Carbon dioxide Plasma membrane intrinsic protein 2
MeSH Terms
Amino Acid Sequence Animals Aquaporins/genetics,metabolism Biological Transport Carbon Dioxide/metabolism Cell Membrane/metabolism Cell Membrane Permeability Cytosol/metabolism Gene Expression Gene Expression Regulation, Plant Hordeum/genetics,metabolism Hydrogen-Ion Concentration Isoleucine Models, Molecular Molecular Sequence Data Oocytes Plant Proteins/genetics,metabolism Plant Roots/genetics,metabolism Sequence Alignment Water/metabolism Xenopus
Chemicals
Aquaporins Plant Proteins Isoleucine Water Carbon Dioxide
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Mori Izumi C
Institute of Plant Science and Resources, Okayama University, 2-20-1 Chuo, Kurashiki, 710-0046 Japan.
Rhee Jiye
Shibasaka Mineo
Sasano Shizuka
Kaneko Toshiyuki
Horie Tomoaki
Katsuhara Maki
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Article Info
Journal
Plant & cell physiology
Abbr.
Plant Cell Physiol
ISSN
1471-9053
DOI
10.1093/pcp/pcu003
Published
2014-02-00
Epub
2014-00-08
Pages
251-7
Language
English
Region
Japan
NLM ID
9430925
PMCID
PMC3913445
Subset
IM
External Links
PubMed source DOI: 10.1093/pcp/pcu003 PMC Full Text View journal details
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