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PMID: 24403257 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Expression profiling of cervical cancers in Indian women at different stages to identify gene signatures during progression of the disease.

Cancer medicine ·Vol. 2 ·No. 6 ·2013-12-00 ·Pages 836-48

Thomas A, Mahantshetty U, Kannan S, Deodhar K, Shrivastava SK, Kumar-Sinha C, Mulherkar R

Abstract

Cervical cancer is the second most common cancer among women worldwide, with developing countries accounting for >80% of the disease burden. Although in the West, active screening has been instrumental in reducing the incidence of cervical cancer, disease management is hampered due to lack of biomarkers for disease progression and defined therapeutic targets. Here we carried out gene expression profiling of 29 cervical cancer tissues from Indian women, spanning International Federation of Gynaecology and Obstetrics (FIGO) stages of the disease from early lesion (IA and IIA) to progressive stages (IIB and IIIA-B), and identified distinct gene expression signatures. Overall, metabolic pathways, pathways in cancer and signaling pathways were found to be significantly upregulated, while focal adhesion, cytokine-cytokine receptor interaction and WNT signaling were downregulated. Additionally, we identified candidate biomarkers of disease progression such as SPP1, proliferating cell nuclear antigen (PCNA), STK17A, and DUSP1 among others that were validated by quantitative real-time polymerase chain reaction (qRT-PCR) in the samples used for microarray studies as well in an independent set of 34 additional samples. Integrative analysis of our results with other cervical cancer profiling studies could facilitate the development of multiplex diagnostic markers of cervical cancer progression.

Keywords
Biomarker cervical cancer expression profiling microarray real-time PCR
MeSH Terms
Biomarkers, Tumor/genetics Disease Progression Female Gene Expression Profiling Humans India Neoplasm Staging Oligonucleotide Array Sequence Analysis RNA, Messenger/genetics Uterine Cervical Neoplasms/genetics,pathology
Chemicals
Biomarkers, Tumor RNA, Messenger
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Thomas Asha
Advanced Centre for Treatment, Research and Education in Cancer (ACTREC), Tata Memorial Centre, Navi Mumbai, Maharashtra, India.
Mahantshetty Umesh
Kannan Sadhana
Deodhar Kedar
Shrivastava Shyam K
Kumar-Sinha Chandan
Mulherkar Rita
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Article Info
Journal
Cancer medicine
Abbr.
Cancer Med
ISSN
2045-7634
DOI
10.1002/cam4.152
Published
2013-12-00
Epub
2013-00-31
Pages
836-48
Language
English
Region
United States
NLM ID
101595310
PMCID
PMC3892388
Subset
IM
Databases
GEO
GSE46857
External Links
PubMed source DOI: 10.1002/cam4.152 PMC Full Text View journal details
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