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PMID: 2439901 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Neutralizing monoclonal antibody against ras oncogene product p21 which impairs guanine nucleotide exchange.

Molecular and cellular biology ·Vol. 7 ·No. 5 ·1987-05-00 ·Pages 1999-2002

Hattori S, Clanton DJ, Satoh T, Nakamura S, Kaziro Y, Kawakita M, Shih TY

Abstract

The neutralizing monoclonal antibody Y13-259 severely hampers the nucleotide exchange reaction between p21-bound and exogenous guanine nucleotides but does not interfere with the association of GDP to p21. These results suggest that the nucleotide exchange reaction is critical for p21 function. Interestingly, the v-ras p21 has a much faster dissociation rate than the p21 of the c-ras proto-oncogene.

MeSH Terms

Antibodies, Monoclonal/immunology Antigen-Antibody Reactions Cell Transformation, Neoplastic/genetics Epitopes GTP-Binding Proteins/immunology,metabolism Guanine Nucleotides/metabolism Neutralization Tests Oncogene Proteins, Viral/immunology,metabolism Oncogenes Protein Binding Proto-Oncogene Proteins/immunology,metabolism

Chemicals

Antibodies, Monoclonal Epitopes Guanine Nucleotides Oncogene Proteins, Viral Proto-Oncogene Proteins GTP-Binding Proteins

Authors & Affiliations

7 authors, click to expand affiliations / ORCID

Hattori S

Clanton D J

Satoh T

Nakamura S

Kaziro Y

Kawakita M

Shih T Y

References (27)

27 references, click to expand

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Article Info

Journal

Molecular and cellular biology

Abbr.

Mol Cell Biol

ISSN

0270-7306

DOI

10.1128/mcb.7.5.1999-2002.1987

Published

1987-05-00

Pages

1999-2002

Language

English

Region

United States

NLM ID

8109087

PMCID

PMC365309

Subset

External Links

PubMed source DOI: 10.1128/mcb.7.5.1999-2002.1987 PMC Full Text View journal details

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Neutralizing monoclonal antibody against ras oncogene product p21 which impairs guanine nucleotide exchange.

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