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PMID: 24362565 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Architecture of the large subunit of the mammalian mitochondrial ribosome.

Nature ·Vol. 505 ·No. 7484 ·2014-01-23 ·Pages 515-9

Greber BJ, Boehringer D, Leitner A, Bieri P, Voigts-Hoffmann F, Erzberger JP, Leibundgut M, Aebersold R, Ban N

Abstract

Mitochondrial ribosomes synthesize a number of highly hydrophobic proteins encoded on the genome of mitochondria, the organelles in eukaryotic cells that are responsible for energy conversion by oxidative phosphorylation. The ribosomes in mammalian mitochondria have undergone massive structural changes throughout their evolution, including ribosomal RNA shortening and acquisition of mitochondria-specific ribosomal proteins. Here we present the three-dimensional structure of the 39S large subunit of the porcine mitochondrial ribosome determined by cryo-electron microscopy at 4.9 Å resolution. The structure, combined with data from chemical crosslinking and mass spectrometry experiments, reveals the unique features of the 39S subunit at near-atomic resolution and provides detailed insight into the architecture of the polypeptide exit site. This region of the mitochondrial ribosome has been considerably remodelled compared to its bacterial counterpart, providing a specialized platform for the synthesis and membrane insertion of the highly hydrophobic protein components of the respiratory chain.

MeSH Terms
Animals Cattle Cryoelectron Microscopy Hydrophobic and Hydrophilic Interactions Mass Spectrometry Mitochondria/chemistry,ultrastructure Mitochondrial Proteins/chemistry,ultrastructure Models, Molecular Nucleic Acid Conformation Protein Conformation RNA, Ribosomal, 16S/chemistry,ultrastructure Ribosomal Proteins/chemistry,ultrastructure Ribosome Subunits/chemistry,ultrastructure Swine
Chemicals
Mitochondrial Proteins RNA, Ribosomal, 16S Ribosomal Proteins
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Greber Basil J
1] Department of Biology, Institute of Molecular Biology and Biophysics, Schafmattstrasse 20, ETH Zurich, CH-8093 Zurich, Switzerland [2].
Boehringer Daniel
1] Department of Biology, Institute of Molecular Biology and Biophysics, Schafmattstrasse 20, ETH Zurich, CH-8093 Zurich, Switzerland [2].
Leitner Alexander
Department of Biology, Institute of Molecular Systems Biology, Wolfgang-Pauli-Strasse 16, ETH Zurich, CH-8093 Zurich, Switzerland.
Bieri Philipp
Department of Biology, Institute of Molecular Biology and Biophysics, Schafmattstrasse 20, ETH Zurich, CH-8093 Zurich, Switzerland.
Voigts-Hoffmann Felix
Department of Biology, Institute of Molecular Biology and Biophysics, Schafmattstrasse 20, ETH Zurich, CH-8093 Zurich, Switzerland.
Erzberger Jan P
Department of Biology, Institute of Molecular Biology and Biophysics, Schafmattstrasse 20, ETH Zurich, CH-8093 Zurich, Switzerland.
Leibundgut Marc
Department of Biology, Institute of Molecular Biology and Biophysics, Schafmattstrasse 20, ETH Zurich, CH-8093 Zurich, Switzerland.
Aebersold Ruedi
1] Department of Biology, Institute of Molecular Systems Biology, Wolfgang-Pauli-Strasse 16, ETH Zurich, CH-8093 Zurich, Switzerland [2] Faculty of Science, University of Zurich, CH-8057 Zurich, Switzerland.
Ban Nenad
Department of Biology, Institute of Molecular Biology and Biophysics, Schafmattstrasse 20, ETH Zurich, CH-8093 Zurich, Switzerland.
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Article Info
Journal
Nature
Abbr.
Nature
ISSN
1476-4687
DOI
10.1038/nature12890
Published
2014-01-23
Epub
2013-00-22
Pages
515-9
Language
English
Region
England
NLM ID
0410462
Subset
IM
Databases
PDB
4CE4
External Links
PubMed source DOI: 10.1038/nature12890 View journal details
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