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PMID: 2435546 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Differential nuclease sensitivity identifies tight contacts between yeast pre-mRNA and spliceosomes.

The EMBO journal ·Vol. 5 ·No. 13 ·1986-12-20 ·Pages 3517-23

Rymond BC, Rosbash M

Abstract

The oligonucleotide-directed RNase H sensitivity of a yeast (Saccharomyces cerevisiae) pre-mRNA was determined in an in vitro splicing reaction. While most of the pre-mRNA was sensitive to cleavage, the regions of the 5' splice site and TACTAAC box were found to be highly resistant. The biochemical requirements for protection against nuclease attack parallel those of both spliceosome formation and splicing. Most of the uncleaved pre-mRNA remaining after RNase H challenge was found associated with two forms of the yeast spliceosome. Differences in the RNase H sensitivity of pre-mRNA found in the two spliceosome forms indicate an increased association of splicing factors with the 5' splice site during spliceosome assembly.

MeSH Terms
Base Sequence Endoribonucleases Nucleic Acid Precursors/genetics Plasmids RNA Precursors RNA Splicing RNA, Catalytic RNA, Messenger/genetics RNA, Ribosomal/genetics RNA-Directed DNA Polymerase Ribonuclease H Saccharomyces cerevisiae/genetics
Chemicals
Nucleic Acid Precursors RNA Precursors RNA, Catalytic RNA, Messenger RNA, Ribosomal RNA-Directed DNA Polymerase Endoribonucleases Ribonuclease H
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Rymond B C
Rosbash M
References (24)
24 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1986-12-20
Pages
3517-23
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1167388
Subset
IM
Grants
NIGMS NIH HHS · GM23549 · United States
External Links
PubMed source PMC Full Text View journal details
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