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PMID: 2433293 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Phasic release of newly synthesized secretory proteins in the unstimulated rat exocrine pancreas.

The Journal of cell biology ·Vol. 104 ·No. 2 ·1987-02-00 ·Pages 243-52

Arvan P, Castle JD

Abstract

Pancreatic lobules from fasted rats secrete pulse-labeled proteins in two phases comprising 15 and 85% of basal output, respectively. The first (0-6.5 h) is initially (less than or equal to 0.5 h) unstimulated by secretagogues, probably represents vesicular traffic of Golgi and post-Golgi origin (including condensing vaculoles/immature granules), and notably contains two groups of polypeptides with distinct release rates: zymogens (t1/2 approximately 2.4 h) and minor nonzymogens plus one unique zymogen (t1/2 approximately 1 h). The second phase (peak at 9-10 h) is stimulable, probably represents basal granule exocytosis (t1/2 approximately 5 h), and contains zymogens exclusively. Newly synthesized proteins released in both phases appear asynchronously, reiterating their asynchronous transport through intracellular compartments. Zymogens in both phases are secreted apically. The sorting of first from second phase zymogen release does not appear to be carrier-mediated, although the sorting of zymogens from other secretory proteins may use this process. Finally, data are presented that suggest that both secretory phases are subject to physiologic regulation.

MeSH Terms
Amylases/biosynthesis,metabolism Animals Chymotrypsinogen/biosynthesis,metabolism Cytoplasmic Granules/metabolism Endoplasmic Reticulum/metabolism Enzyme Precursors/biosynthesis,isolation & purification,metabolism Exocytosis Golgi Apparatus/metabolism In Vitro Techniques Kinetics Lipase/biosynthesis,metabolism Male Molecular Weight Pancreas/enzymology,metabolism Pancreatic Hormones/biosynthesis,isolation & purification,metabolism Rats Rats, Inbred Strains Trypsinogen/biosynthesis,metabolism
Chemicals
Enzyme Precursors Pancreatic Hormones Trypsinogen Chymotrypsinogen Lipase Amylases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Arvan P
Castle J D
References (34)
34 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.104.2.243
Published
1987-02-00
Pages
243-52
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2114415
Subset
IM
Grants
NIGMS NIH HHS · GM-26324 · United States
External Links
PubMed source DOI: 10.1083/jcb.104.2.243 PMC Full Text View journal details
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