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PMID: 24329902 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Overexpression of a proton-coupled vacuolar glucose exporter impairs freezing tolerance and seed germination.

The New phytologist ·Vol. 202 ·No. 1 ·2014-04-00 ·Pages 188-197

Klemens PAW, Patzke K, Trentmann O, Poschet G, Büttner M, Schulz A, Marten I, Hedrich R, Neuhaus HE

Abstract

Arabidopsis vacuoles harbor, besides sugar transporter of the TMT-type, an early response to dehydration like 6 (ERDL6) protein involved in glucose export into the cytosol. However, the mode of transport of ERDL6 and the plant's feedback to overexpression of its activity on essential properties such as, for example, seed germination or freezing tolerance, remain unexplored. Using patch-clamp studies on vacuoles expressing AtERDL6 we demonstrated directly that this carrier operates as a proton-driven glucose exporter. Overexpression of BvIMP, the closest sugar beet (Beta vulgaris) homolog to AtERDL6, in Arabidopsis leads surprisingly to impaired seed germination under both conditions, sugar application and low environmental temperatures, but not under standard conditions. Upon cold treatment, BvIMP overexpressor plants accumulated lower quantities of monosaccharides than the wild-type, a response in line with the reduced frost tolerance of the transgenic Arabidopsis plants, and the fact that cold temperatures inhibits BvIMP transcription in sugar beet leaves. With these findings we show that the tight control of vacuolar sugar import and export is a key requisite for cold tolerance and seed germination of plants.

Keywords
Arabidopsis glucose transport stress tolerance tonoplast vacuole
MeSH Terms
Adaptation, Physiological Arabidopsis/genetics,physiology Arabidopsis Proteins/genetics,metabolism Beta vulgaris Biocatalysis Biological Transport Carbohydrate Metabolism Electric Conductivity Freezing Gene Expression Regulation, Plant Germination Glucose/metabolism Light-Harvesting Protein Complexes/genetics,metabolism Monosaccharide Transport Proteins/genetics,metabolism Photosystem II Protein Complex/genetics,metabolism Plant Proteins/genetics,metabolism Plants, Genetically Modified Protons RNA, Messenger/genetics,metabolism Seeds/genetics,growth & development Signal Transduction Starch/metabolism Vacuoles/metabolism
Chemicals
Arabidopsis Proteins CAB1 protein, Arabidopsis Light-Harvesting Protein Complexes Monosaccharide Transport Proteins Photosystem II Protein Complex Plant Proteins Protons RNA, Messenger Starch Glucose
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Klemens Patrick A W
Plant Physiology, University of Kaiserslautern, Erwin-Schrödinger-Str., D-67653, Kaiserslautern, Germany.
Patzke Kathrin
Plant Physiology, University of Kaiserslautern, Erwin-Schrödinger-Str., D-67653, Kaiserslautern, Germany.
Trentmann Oliver
Plant Physiology, University of Kaiserslautern, Erwin-Schrödinger-Str., D-67653, Kaiserslautern, Germany.
Poschet Gernot
Centre for Organismal Studies (COS), University of Heidelberg, Im Neuenheimer Feld 360, 69120, Heidelberg, Germany.
Büttner Michael
Centre for Organismal Studies (COS), University of Heidelberg, Im Neuenheimer Feld 360, 69120, Heidelberg, Germany.
Schulz Alexander
Institute for Molecular Plant Physiology and Biophysics, University of Würzburg, Julius-von-Sachs Platz 2, D-97082, Würzburg, Germany.
Marten Irene
Institute for Molecular Plant Physiology and Biophysics, University of Würzburg, Julius-von-Sachs Platz 2, D-97082, Würzburg, Germany.
Hedrich Rainer
Institute for Molecular Plant Physiology and Biophysics, University of Würzburg, Julius-von-Sachs Platz 2, D-97082, Würzburg, Germany.
Neuhaus H Ekkehard
Plant Physiology, University of Kaiserslautern, Erwin-Schrödinger-Str., D-67653, Kaiserslautern, Germany.
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Article Info
Journal
The New phytologist
Abbr.
New Phytol
ISSN
1469-8137
DOI
10.1111/nph.12642
Published
2014-04-00
Epub
2013-00-16
Pages
188-197
Language
English
Region
England
NLM ID
9882884
Subset
IM
External Links
PubMed source DOI: 10.1111/nph.12642 View journal details
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