A detailed restriction endonuclease site map of theZea mays plastid genome.

Plant molecular biology ·Vol. 2 ·No. 3 ·1983-05-00 ·Pages 129-40

Larrinua IM, Muskavitch KM, Gubbins EJ, Bogorad L

Abstract

Fragments produced by partial digestion of plastid DNA fromZea mays withEco RI were cloned in Charon 4A. A circular, fine structure physical map of the plastid DNA was then constructed from restriction endonucleaseSal I,Pst I,Eco RI, andBam HI recognition site maps of cloned overlapping segments of the plastid genome. These fragments were assigned molecular weights by reference to size markers from both pBR322 and lambda phage DNA. Because of the detail and extent of the derived map, it has been possible to construct a coordinate system which has a unique zero point and within which all the restriction fragments and previously described structural features can be mapped. A computer program was constructed which will display in a circular fashion any of the above features using an X-Y plotter.

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Larrinua I M

The Biological Laboratories, Harvard University, 02138, Cambridge, MA, USA.

Muskavitch K M

Gubbins E J

Bogorad L

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Article Info

Journal

Plant molecular biology

Abbr.

Plant Mol Biol

ISSN

0167-4412

DOI

10.1007/BF01578513

Published

1983-05-00

Pages

129-40

Language

English

Region

Netherlands

NLM ID

9106343

External Links

PubMed source DOI: 10.1007/BF01578513 View journal details

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A detailed restriction endonuclease site map of theZea mays plastid genome.

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