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题目:: High-throughput synchronization of mammalian cell cultures by spiral microfluidics.
作者:: Lee(Wong Cheng),Bhagat(Ali Asgar S),Lim(Chwee Teck)
状态:: 发布时间2013-12-03 , 更新时间 2013-12-03
期刊:: Methods Mol Biol
摘要:: The development of mammalian cell cycle synchronization techniques has greatly advanced our understanding of many cellular regulatory events and mechanisms specific to different phases of the cell cycle. In this chapter, we describe a high-throughput microfluidic-based approach for cell cycle synchronization. By exploiting the relationship between cell size and its phase in the cell cycle, large numbers of synchronized cells can be obtained by size fractionation in a spiral microfluidic channel. Protocols for the synchronization of primary cells such as mesenchymal stem cells, and immortal cell lines such as Chinese hamster ovarian cells (CHO-CD36) and HeLa cells are provided as examples.
语言:: eng
DOI:: 10.1007/978-1-62703-733-4_1

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