Home LiteratureArticle Details
PMID: 2428841 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Divalent cation regulation of the surface orientation of platelet membrane glycoprotein IIb. Correlation with fibrinogen binding function and definition of a novel variant of Glanzmann's thrombasthenia.

The Journal of clinical investigation ·Vol. 78 ·No. 4 ·1986-10-00 ·Pages 1103-11

Ginsberg MH, Lightsey A, Kunicki TJ, Kaufmann A, Marguerie G, Plow EF

Abstract

An antiplatelet monoclonal antibody, PMI-1, reacts with glycoproteins (GP) GPIIb, free GPIIb, and the GPIIb-IIIa complex. This antibody binds to 40,900 sites per platelet, with a Kd = 0.95 microM, and its binding is inhibited by the presence of magnesium or calcium in the suspending medium (50% suppression at approximately 0.5 mM divalent cation). Regulation of the PMI-1 epitope is independent of disassembly of the GPIIb-IIIa heterodimer, because it occurred at 22 degrees C and in response to mM magnesium as well as calcium. PMI-1 binding inversely correlated with fibrinogen binding. In addition, we identified a variant of Glanzmann's thrombasthenia with near-normal platelet content of the GPIIb-IIIa heterodimer as judged by crossed immunoelectrophoresis and surface labeling. Binding of PMI-1 to these patients' platelets was not dependent on reduction of the divalent cation concentration. These data suggest that the surface orientation of GPIIb is important in the capacity of platelets to bind fibrinogen.

MeSH Terms
Antibodies, Monoclonal Antibody Specificity Blood Platelet Disorders/metabolism Blood Platelets/immunology Cations, Divalent/pharmacology Epitopes/analysis Fibrinogen/metabolism Humans Immunoelectrophoresis, Two-Dimensional Kinetics Platelet Aggregation Platelet Membrane Glycoproteins/metabolism Thrombasthenia/metabolism
Chemicals
Antibodies, Monoclonal Cations, Divalent Epitopes Platelet Membrane Glycoproteins Fibrinogen
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Ginsberg M H
Lightsey A
Kunicki T J
Kaufmann A
Marguerie G
Plow E F
References (41)
41 references, click to expand
  1. Factors responsible for ADP-induced release reaction of human platelets.
    Am J Physiol. 1975 Jun;228(6):1757-65 PMID: 50744
  2. Calcium regulation of the platelet membrane glycoprotein IIb-IIIa complex.
    J Biol Chem. 1985 Sep 15;260(20):11366-74 PMID: 3161896
  3. Radioiodination of proteins in single polyacrylamide gel slices. Tryptic peptide analysis of all the major members of complex multicomponent systems using microgram quantities of total protein.
    J Biol Chem. 1977 Sep 25;252(18):6510-5 PMID: 893422
  4. The binding of calcium to fibrinogen: some structural features.
    Biochim Biophys Acta. 1977 Sep 27;494(1):172-81 PMID: 20154
  5. Human platelets possess an inducible and saturable receptor specific for fibrinogen.
    J Biol Chem. 1979 Jun 25;254(12):5357-63 PMID: 447655
  6. The different glycoprotein abnormalities in thrombasthenic and Bernard-Soulier platelets.
    Semin Hematol. 1979 Jul;16(3):234-50 PMID: 384525
  7. Comparison of fibrinogen association with normal and thrombasthenic platelets on exposure to ADP or chymotrypsin.
    Blood. 1979 Nov;54(5):987-93 PMID: 497404
  8. Exposure of platelet fibrinogen receptors by ADP and epinephrine.
    J Clin Invest. 1979 Nov;64(5):1393-401 PMID: 574143
  9. Interaction of fibrinogen with its platelet receptor as part of a multistep reaction in ADP-induced platelet aggregation.
    J Biol Chem. 1980 Jan 10;255(1):154-61 PMID: 7350149
  10. Immunochemical evidence for protein abnormalities in platelets from patients with Glanzmann's thrombasthenia and Bernard-Soulier syndrome.
    J Clin Invest. 1980 Mar;65(3):722-31 PMID: 7354135
  11. Correlation between fibrinogen binding to human platelets and platelet aggregability.
    Blood. 1980 May;55(5):841-7 PMID: 6767512
  12. Ligand: a versatile computerized approach for characterization of ligand-binding systems.
    Anal Biochem. 1980 Sep 1;107(1):220-39 PMID: 6254391
  13. Isolation and quantitation of the platelet membrane glycoprotein deficient in thrombasthenia using a monoclonal hybridoma antibody.
    J Clin Invest. 1980 Dec;66(6):1311-8 PMID: 6449521
  14. Heterogeneity of membrane surface proteins in Glanzmann's thrombasthenia.
    Blood. 1981 Jan;57(1):174-81 PMID: 7448409
  15. Isolation, purification, and partial characterization of platelet membrane glycoproteins IIb and IIIa.
    J Biol Chem. 1981 Feb 25;256(4):1994-7 PMID: 6450761
  16. Relationship between fibrinogen binding and the platelet glycoprotein deficiencies in Glanzmann's thrombasthenia type I and type II.
    Br J Haematol. 1981 May;48(1):47-57 PMID: 6454435
  17. The formation of Ca++-dependent complexes of platelet membrane glycoproteins IIb and IIIa in solution as determined by crossed immunoelectrophoresis.
    Blood. 1981 Aug;58(2):268-78 PMID: 6454451
  18. Isolation and structural characterization of the polypeptide subunits of membrane glycoprotein IIb-IIIa from human platelets.
    Blood. 1982 Jan;59(1):80-5 PMID: 7053767
  19. The role of cell surface proteins in platelet stimulation by monosodium urate crystals.
    Arthritis Rheum. 1982 May;25(5):508-21 PMID: 7082398
  20. Identification of the fibrinogen receptor on human platelets by photoaffinity labeling.
    J Biol Chem. 1982 Jul 25;257(14):8049-54 PMID: 6282870
  21. Fibrinogen-binding properties of the human platelet glycoprotein IIb-=IIIa complex: a study using crossed-radioimmunoelectrophoresis.
    Blood. 1982 Sep;60(3):663-71 PMID: 6286013
  22. Purification of glycoproteins IIb and III from human platelet plasma membranes and characterization of a calcium-dependent glycoprotein IIb-III complex.
    J Biol Chem. 1982 Sep 10;257(17):10458-66 PMID: 6213621
  23. Exposure of platelet fibrinogen-binding sites by collagen, arachidonic acid, and ADP: inhibition by a monoclonal antibody to the glycoprotein IIb-IIIa complex.
    Blood. 1983 Jan;61(1):140-8 PMID: 6216929
  24. Reduced surface expression and binding of fibronectin by thrombin-stimulated thrombasthenic platelets.
    J Clin Invest. 1983 Mar;71(3):619-24 PMID: 6826726
  25. Identification of two structurally and functionally distinct sites on human platelet membrane glycoprotein IIb-IIIa using monoclonal antibodies.
    J Biol Chem. 1983 Apr 25;258(8):5269-75 PMID: 6220015
  26. Inhibition of fibrinogen binding to stimulated human platelets by a monoclonal antibody.
    Proc Natl Acad Sci U S A. 1983 May;80(9):2417-21 PMID: 6302680
  27. Mechanisms of postprandial glucose counterregulation in man. Physiologic roles of glucagon and epinephrine vis-a-vis insulin in the prevention of hypoglycemia late after glucose ingestion.
    J Clin Invest. 1983 Jul;72(1):278-86 PMID: 6135707
  28. Fibrinogen binding to human platelet plasma membranes. Identification of two steps requiring divalent cations.
    J Biol Chem. 1983 Sep 10;258(17):10240-6 PMID: 6309797
  29. Calcium cation regulation of glycoprotein IIb-IIIa complex formation in platelet plasma membranes.
    J Biol Chem. 1983 Sep 10;258(17):10247-52 PMID: 6224787
  30. Binding of fibronectin to alpha-granule-deficient platelets.
    J Cell Biol. 1983 Aug;97(2):571-3 PMID: 6309863
  31. Interaction of AP-2, a monoclonal antibody specific for the human platelet glycoprotein IIb-IIIa complex, with intact platelets.
    J Biol Chem. 1983 Oct 25;258(20):12582-6 PMID: 6226659
  32. Interaction of human platelet membrane glycoproteins with collagen and lectins.
    Biochim Biophys Acta. 1984 Jan 24;797(1):10-9 PMID: 6229286
  33. Complex formation of platelet membrane glycoproteins IIb and IIIa: a model of platelet activation.
    Bibl Haematol. 1983;(49):127-30 PMID: 6320793
  34. The platelet-fibrinogen interaction. Evidence for proximity of the A alpha chain of fibrinogen to platelet membrane glycoproteins IIb/III.
    Eur J Biochem. 1984 Feb 15;139(1):5-11 PMID: 6230229
  35. Identification and function of the high affinity binding sites for Ca2+ on the surface of platelets.
    J Clin Invest. 1984 Mar;73(3):626-32 PMID: 6231306
  36. Interaction of specific platelet membrane proteins with collagen: evidence from chemical cross-linking.
    Biochemistry. 1984 Jun 19;23(13):3099-104 PMID: 6466633
  37. Platelet-collagen adhesion: evidence for participation of antigenically distinct entities.
    J Cell Biol. 1984 Dec;99(6):2048-55 PMID: 6209287
  38. Platelet-collagen adhesion: inhibition by a monoclonal antibody that binds glycoprotein IIb.
    J Cell Biol. 1984 Dec;99(6):2056-60 PMID: 6238974
  39. Reconstruction of platelet proteins into phospholipid vesicles. Functional proteoliposomes.
    J Clin Invest. 1985 Jan;75(1):35-9 PMID: 3155527
  40. Biochemical and functional consequences of dissociation of the platelet membrane glycoprotein IIb-IIIa complex.
    Blood. 1985 Jul;66(1):92-8 PMID: 3159447
  41. Platelet plasma membrane glycoproteins. Evidence for the presence of nonequivalent disulfide bonds using nonreduced-reduced two-dimensional gel electrophoresis.
    J Biol Chem. 1977 Mar 25;252(6):2121-6 PMID: 845165
Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI112667
Published
1986-10-00
Pages
1103-11
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC423772
Subset
IM
Grants
NIADDK NIH HHS · AM-27214 · United States
NHLBI NIH HHS · HL-16411 · United States
NHLBI NIH HHS · HL-32279 · United States
External Links
PubMed source DOI: 10.1172/JCI112667 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2