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PMID: 24284817 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Natural variation in the genes responsible for maturity loci E1, E2, E3 and E4 in soybean.

Annals of botany ·Vol. 113 ·No. 3 ·2014-02-00 ·Pages 429-41

Tsubokura Y, Watanabe S, Xia Z, Kanamori H, Yamagata H, Kaga A, Katayose Y, Abe J, Ishimoto M, Harada K

Abstract

The timing of flowering has a direct impact on successful seed production in plants. Flowering of soybean (Glycine max) is controlled by several E loci, and previous studies identified the genes responsible for the flowering loci E1, E2, E3 and E4. However, natural variation in these genes has not been fully elucidated. The aims of this study were the identification of new alleles, establishment of allele diagnoses, examination of allelic combinations for adaptability, and analysis of the integrated effect of these loci on flowering. The sequences of these genes and their flanking regions were determined for 39 accessions by primer walking. Systematic discrimination among alleles was performed using DNA markers. Genotypes at the E1-E4 loci were determined for 63 accessions covering several ecological types using DNA markers and sequencing, and flowering times of these accessions at three sowing times were recorded. A new allele with an insertion of a long interspersed nuclear element (LINE) at the promoter of the E1 locus (e1-re) was identified. Insertion and deletion of 36 bases in the eighth intron (E2-in and E2-dl) were observed at the E2 locus. Systematic discrimination among the alleles at the E1-E3 loci was achieved using PCR-based markers. Allelic combinations at the E1-E4 loci were found to be associated with ecological types, and about 62-66 % of variation of flowering time could be attributed to these loci. The study advances understanding of the combined roles of the E1-E4 loci in flowering and geographic adaptation, and suggests the existence of unidentified genes for flowering in soybean.

Keywords
E locus Glycine max SNP ecological type flowering time haplotype marker-assisted selection single nucleotide polymorphism soybean
MeSH Terms
Adaptation, Physiological Alleles Base Sequence Chromosome Mapping Flowers/genetics,physiology Gene Expression Regulation, Plant Genetic Loci/genetics Genetic Markers/genetics Genetic Variation Genotype Haplotypes Molecular Sequence Data Photoperiod Plant Proteins/genetics,metabolism Polymorphism, Single Nucleotide Quantitative Trait Loci/genetics Seeds/genetics,physiology Sequence Alignment Sequence Analysis, DNA Soybeans/genetics,physiology Time Factors
Chemicals
Genetic Markers Plant Proteins
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Tsubokura Yasutaka
National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 306-8602, Japan.
Watanabe Satoshi
Xia Zhengjun
Kanamori Hiroyuki
Yamagata Harumi
Kaga Akito
Katayose Yuichi
Abe Jun
Ishimoto Masao
Harada Kyuya
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Article Info
Journal
Annals of botany
Abbr.
Ann Bot
ISSN
1095-8290
DOI
10.1093/aob/mct269
Published
2014-02-00
Epub
2013-00-26
Pages
429-41
Language
English
Region
England
NLM ID
0372347
PMCID
PMC3906962
Subset
IM
Databases
GENBANK
AB795360, AB795361, AB795362, AB795363, AB795364, AB795365, AB795366, AB795367, AB795368, AB795369, AB795370, AB795371, AB795372, AB795373, AB795374, AB795375, AB795376, AB795377, AB795378, AB795379, AB795380, AB795381, AB795382, AB795383, AB795384, AB795385, AB795386, AB795387, AB795388, AB795389, AB795390, AB795391, AB795392, AB797147, AB797148, AB797149, AB797150, AB797151, AB797152, AB797153, AB797154, AB797155, AB797156, AB797157, AB797158, AB797159, AB797160, AB797161, AB797162, AB797163, AB797164, AB797165, AB797166, AB797167, AB797168, AB797169, AB797170, AB797171, AB797172, AB797173, AB797174, AB797175, AB797176, AB797177, AB797178, AB797179, AB797180, AB797181, AB797182, AB797183, AB797184, AB797185, AB797186, AB797187, AB797188, AB797189, AB797190, AB797191, AB797192, AB797193, AB797194, AB797195, AB797196, AB797197, AB797198, AB797199, AB797200, AB797201, AB797202, AB797203, AB797204, AB797205, AB797206, AB797207, AB797208, AB797209, AB797210
External Links
PubMed source DOI: 10.1093/aob/mct269 PMC Full Text View journal details
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