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PMID: 2428012 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The TRP4 gene of Saccharomyces cerevisiae: isolation and structural analysis.

Nucleic acids research ·Vol. 14 ·No. 16 ·1986-08-26 ·Pages 6357-73

Furter R, Paravicini G, Aebi M, Braus G, Prantl F, Niederberger P, Hütter R

Abstract

The TRP4 gene of Saccharomyces cerevisiae, encoding the anthranilate phosphoribosyl transferase, was isolated and subcloned by functional complementation in yeast. A 2 kb fragment containing information for a polypeptide of 380 amino acids and the 5'- and 3'-flanking regions was sequenced. The TRP4 transcript was identified and mapped with S1 nuclease. Homologies to two prokaryotic genes encoding the same function, and sequences potentially involved in transcription start and termination and in regulation of TRP4 gene expression are discussed.

MeSH Terms
Amino Acid Sequence Anthranilate Phosphoribosyltransferase/genetics Base Sequence Cloning, Molecular DNA Restriction Enzymes Endonucleases Genes Genes, Fungal Genetic Complementation Test Pentosyltransferases/genetics Phenotype Poly A/isolation & purification RNA/isolation & purification RNA, Messenger Saccharomyces cerevisiae/genetics Single-Strand Specific DNA and RNA Endonucleases Transcription, Genetic
Chemicals
RNA, Messenger Poly A RNA Pentosyltransferases Anthranilate Phosphoribosyltransferase Endonucleases DNA Restriction Enzymes Single-Strand Specific DNA and RNA Endonucleases
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Furter R
Paravicini G
Aebi M
Braus G
Prantl F
Niederberger P
Hütter R
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/14.16.6357
Published
1986-08-26
Pages
6357-73
Language
English
Region
England
NLM ID
0411011
PMCID
PMC311651
Subset
IM
Databases
GENBANK
X04273
External Links
PubMed source DOI: 10.1093/nar/14.16.6357 PMC Full Text View journal details
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