Conserved epitopes on the hemagglutinin-neuraminidase proteins of human and bovine parainfluenza type 3 viruses: nucleotide sequence analysis of variants selected with monoclonal antibodies.

Journal of virology ·Vol. 60 ·No. 1 ·1986-10-00 ·Pages 90-6

Coelingh KJ, Winter CC, Murphy BR, Rice JM, Kimball PC, Olmsted RA, Collins PL

Abstract

We have previously identified 11 epitopes located in two topologically nonoverlapping antigenic sites (A and B) and a third bridging site (C) on the human type 3 parainfluenza virus (PIV3) hemagglutinin-neuraminidase (HN) glycoprotein by using monoclonal antibodies (MAbs) which inhibit hemagglutination and virus infectivity (K. L. Coelingh, C. C. Winter, and B. R. Murphy, Virology 143:569-582, 1985). We have identified three additional antigenic sites (D, E, and F) on the HN molecule by competitive-binding assays of anti-HN MAbs which have no known biological activity. Epitopes in sites A, D, and F are conserved on the bovine PIV3 HN glycoprotein and also among a wide range of human isolates. The dideoxy method was used to identify nucleotide substitutions in the HN genes of antigenic variants selected with neutralizing MAbs representing epitopes in site A which are shared by human and bovine PIV3. The deduced amino acid substitutions in the variants were located in separate hydrophilic stretches of HN residues which are conserved in the primary structures of the HN proteins of both human and bovine PIV3 strains.

MeSH Terms

Animals Antibodies, Monoclonal/immunology Antibodies, Viral/immunology Antigens, Viral/genetics,immunology Base Sequence Cattle/microbiology Cross Reactions Epitopes/genetics,immunology HN Protein Humans Respirovirus/genetics,immunology Species Specificity Viral Envelope Proteins/genetics,immunology

Chemicals

Antibodies, Monoclonal Antibodies, Viral Antigens, Viral Epitopes HN Protein Viral Envelope Proteins

Authors & Affiliations

7 authors, click to expand affiliations / ORCID

Coelingh K J

Winter C C

Murphy B R

Rice J M

Kimball P C

Olmsted R A

Collins P L

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Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.60.1.90-96.1986

Published

1986-10-00

Pages

90-6

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC253905

Subset

External Links

PubMed source DOI: 10.1128/JVI.60.1.90-96.1986 PMC Full Text View journal details

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Conserved epitopes on the hemagglutinin-neuraminidase proteins of human and bovine parainfluenza type 3 viruses: nucleotide sequence analysis of variants selected with monoclonal antibodies.

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