The tomato Cab-4 and Cab-5 genes encode a second type of CAB polypeptides localized in Photosystem II.

Plant molecular biology ·Vol. 9 ·No. 2 ·1987-03-00 ·Pages 109-20

Pichersky E, Hoffman NE, Malik VS, Bernatzky R, Tanksley SD, Szabo L, Cashmore AR

Abstract

The photosynthetic apparatus of plant chloroplasts contains two photosystems, termed Photosystem I (PSI) and Photosystem II (PSII). Both PSI and PSII contain several types of chlorophyll a/b-binding (CAB) polypeptides, at least some of which are structurally related. It has been previously shown that multiple genes encoding one type of PSII CAB polypeptides exist in the genome of many higher plants. In tomato, there are at least eight such genes, distributed in three independent loci. Genes encoding a second type of CAB polypeptides have been isolated from several plant species, but the precise location of the gene products has not been determined. Here we show that tomato has two unlinked genes encoding this second type and that this type of CAB polypeptide is also localized in PSII.

Authors & Affiliations

7 authors, click to expand affiliations / ORCID

Pichersky E

Laboratory of Cell Biology, The Rockefeller University, 10021, New York, NY, USA.

Hoffman N E

Malik V S

Bernatzky R

Tanksley S D

Szabo L

Cashmore A R

References (29)

29 references, click to expand

Nucleotide sequences of two pea cDNA clones encoding the small subunit of ribulose 1,5-bisphosphate carboxylase and the major chlorophyll a/b-binding thylakoid polypeptide.
J Biol Chem. 1983 Feb 10;258(3):1399-402 PMID: 6296093
Monoclonal antibodies to the light-harvesting chlorophyll a/b protein complex of photosystem II.
J Cell Biol. 1986 Sep;103(3):733-40 PMID: 3528171
Molecular characterization and genetic mapping of two clusters of genes encoding chlorophyll a/b-binding proteins in Lycopersicon esculentum (tomato).
Gene. 1985;40(2-3):247-58 PMID: 3007291
Sequencing end-labeled DNA with base-specific chemical cleavages.
Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
Structure and developmental regulation of a wheat gene encoding the major chlorophyll a/b-binding polypeptide.
Mol Cell Biol. 1985 Jun;5(6):1370-8 PMID: 3897835
A cDNA clone encoding a photosystem I protein with homology to photosystem II chlorophyll a/b-binding polypeptides.
Proc Natl Acad Sci U S A. 1987 Dec;84(24):8844-8 PMID: 3321058
Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
Nucleic Acids Res. 1984 Sep 25;12(18):7035-56 PMID: 6091052
Phytochrome control of RNA levels in developing pea and mung-bean leaves.
Planta. 1983 Aug;158(6):487-500 PMID: 24264922
Chloroplast Import of Light-Harvesting Chlorophyll a/b-Proteins with Different Amino Termini and Transit Peptides.
Plant Physiol. 1986 Dec;82(4):1172-4 PMID: 16665159
Functional and mutational analysis of the light-harvesting chlorophyll a/b protein of thylakoid membranes.
J Cell Biol. 1986 Mar;102(3):972-81 PMID: 3512583
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
A chlorophyll a/b-protein encoded by a gene containing an intron with characteristics of a transposable element.
J Mol Appl Genet. 1985;3(1):45-61 PMID: 2989405
Cloned DNA sequences complementary to mRNAs encoding precursors to the small subunit of ribulose-1,5-bisphosphate carboxylase and a chlorophyll a/b binding polypeptide.
Proc Natl Acad Sci U S A. 1981 Dec;78(12):7304-8 PMID: 16593132
The petunia chlorophyll a/b binding protein genes: a comparison of Cab genes from different gene families.
Nucleic Acids Res. 1985 Apr 11;13(7):2503-18 PMID: 2987856
Functional determinants in transit sequences: import and partial maturation by vascular plant chloroplasts of the ribulose-1,5-bisphosphate carboxylase small subunit of Chlamydomonas.
J Cell Biol. 1985 Jan;100(1):226-34 PMID: 3965471
Silene cDNA clones for a divergent chlorophyll-a/b-binding protein and a small subunit of ribulosebisphosphate carboxylase.
Plant Mol Biol. 1986 Nov;7(6):433-40 PMID: 24302471
Chlorophyll proteins of photosystem I.
Plant Physiol. 1980 May;65(5):814-22 PMID: 16661288
A simplified and efficient vector-primer cDNA cloning system.
Gene. 1984 Nov;31(1-3):79-89 PMID: 6098539
Biosynthetic pathways of two polypeptide subunits of the light-harvesting chlorophyll a/b protein complex.
J Cell Biol. 1981 Nov;91(2 Pt 1):468-78 PMID: 7031068
Optimal conditions for post-translational uptake of proteins by isolated chloroplasts. In vitro synthesis and transport of plastocyanin, ferredoxin-NADP+ oxidoreductase, and fructose-1,6-bisphosphatase.
J Biol Chem. 1982 Feb 10;257(3):1558-63 PMID: 6276392
Structure and expression of a pea nuclear gene encoding a chlorophyll a/b-binding polypeptide.
Proc Natl Acad Sci U S A. 1984 May;81(10):2960-4 PMID: 16593461
Toward a saturated linkage map in tomato based on isozymes and random cDNA sequences.
Genetics. 1986 Apr;112(4):887-98 PMID: 17246322
A novel chlorophyll a/b binding (Cab) protein gene from petunia which encodes the lower molecular weight Cab precursor protein.
Nucleic Acids Res. 1986 Dec 22;14(24):9781-96 PMID: 3027663
Structure and expression of three light-harvesting chlorophyll a/b-binding protein genes in Arabidopsis thaliana.
Nucleic Acids Res. 1986 May 27;14(10):4051-64 PMID: 3012462
Cell-free translation of messenger RNA in a wheat germ system.
Methods Enzymol. 1983;96:38-50 PMID: 6656637
Localization in the Tomato Genome of DNA Restriction Fragments Containing Sequences Homologous to the rRNA (45s), the Major Chlorophyll a/b Binding Polypeptide and the Ribulose Bisphosphate Carboxylase Genes.
Genetics. 1986 Jan;112(1):93-105 PMID: 17246311
Precursors to two nuclear-encoded chloroplast proteins bind to the outer envelope membrane before being imported into chloroplasts.
J Biol Chem. 1985 Mar 25;260(6):3691-6 PMID: 3882712
The major chlorophyll a/b binding protein of petunia is composed of several polypeptides encoded by a number of distinct nuclear genes.
J Mol Appl Genet. 1983;2(3):285-300 PMID: 6319526
Post-translational transport into intact chloroplasts of a precursor to the small subunit of ribulose-1,5-bisphosphate carboxylase.
Proc Natl Acad Sci U S A. 1978 Dec;75(12):6110-4 PMID: 16592597

Article Info

Journal

Plant molecular biology

Abbr.

Plant Mol Biol

ISSN

0167-4412

DOI

10.1007/BF00015643

Published

1987-03-00

Pages

109-20

Language

English

Region

Netherlands

NLM ID

9106343

External Links

PubMed source DOI: 10.1007/BF00015643 View journal details

Analysis Services

The tomato Cab-4 and Cab-5 genes encode a second type of CAB polypeptides localized in Photosystem II.

Contact

WeChat Official Account