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PMID: 24272344 Published · ppublish English Journal Article

Localization and nucleotide sequence of the gene for the 8 kDa subunit of photosystem I in pea and wheat chloroplast DNA.

Plant molecular biology ·Vol. 11 ·No. 3 ·1988-05-00 ·Pages 311-9

Dunn PP, Gray JC

Abstract

The gene for the 8 kDa subunit of photosystem I has been located in the small single copy region of wheat chloroplast DNA by coupled transcription-translation of cloned fragments of DNA and by DNA sequence analysis. The pea gene for this subunit was located in pea chloroplast DNA by using the wheat gene as a probe. The location was confirmed by immunoprecipitation of the products of coupled transcription-translation of cloned DNA with antiserum raised against the small subunits of pea photosystem I and by DNA sequence analysis. The deduced amino acid sequences of the pea and wheat proteins are identical in seventy-six out of the eighty-one amino acid residues. There are nine conserved cysteine residues, eight of which are arranged in the primary structure in a similar way to those in bacterial ferredoxins containing two 4Fe-4S centres, suggesting that the polypeptide binds iron sulphur centres A and B of photosystem I.

Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Dunn P P
Botany School, University of Cambridge, Downing Street, CB2 3EA, Cambridge, UK.
Gray J C
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Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1007/BF00027388
Published
1988-05-00
Pages
311-9
Language
English
Region
Netherlands
NLM ID
9106343
External Links
PubMed source DOI: 10.1007/BF00027388 View journal details
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