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PMID: 24272156 Published · ppublish English Journal Article

Agroinfection and nucleotide sequence of cloned wheat dwarf virus DNA.

Plant molecular biology ·Vol. 11 ·No. 1 ·1988-01-00 ·Pages 35-43

Woolston CJ, Barker R, Gunn H, Boulton MI, Mullineaux PM

Abstract

Cloned DNA of the geminivirus wheat dwarf virus (WDV) was successfully used to infect seedling wheat plants. The clone was derived from circular double-stranded viral DNA isolated from naturally infected tissue. The initiation of infection was mediated by Agrobacterium tumefaciens using cloned dimeric WDV genomes in a binary Agrobacterium vector. The WDV DNA which comprised the infectious clone was sequenced and is compared with the published sequence of a Swedish isolate of the same virus. The results confirm that the single WDV genome component of 2.75 kb carries all the information necessary for production of viral symptoms, virus particles and viral double- and single-stranded DNA forms.

Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Woolston C J
AFRC Institute of Plant Science Research, John Innes Institute, Colney Lane, NR4 7UH, Norwich, Norfolk, UK.
Barker R
Gunn H
Boulton M I
Mullineaux P M
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Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1007/BF00016012
Published
1988-01-00
Pages
35-43
Language
English
Region
Netherlands
NLM ID
9106343
External Links
PubMed source DOI: 10.1007/BF00016012 View journal details
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