Detection and characterization of a lectin from non-seed tissue ofPhaseolus vulgaris.

Planta ·Vol. 161 ·No. 3 ·1984-05-00 ·Pages 223-8

Borrebaeck CA

Abstract

The distribution of lectin in various tissues ofPhaseolus vulgaris L. (cv. red) has been investigated using a sensitive solid-phase enzyme immunoassay. Roots, leaves and stems from 3- to 4-week-old plants were screened for their lectin content; low levels could be detected in all organs, with a relative distribution of 37% in roots, 20% in leaves and 43% in stems. The lectin from stemsleaves and roots was then isolated from 5- to 6-week-old plants using extraction, salt fractionation and affinity chromatography on immobilized porcine thyroglobulin. A comparative study of the seed lectin and the lectin isolated from 5- to 6-week-old plants was made using hemagglutination, inhibition of hemagglutination, immunodiffusion, polyacrylamide and agarose electrophoresis. The results showed that lectin isolated from the different tissues was immunologically identical and exhibited the same subunit structure and similar isolectin composition as the seed lectin.

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Borrebaeck C A

Department of Pure and Applied Biochemistry, University of Lund, P.O. Box 740, S-220 07, Lund, Sweden.

References (25)

25 references, click to expand

A colorimetric method for monitoring activation of Sepharose by cyanogen bromide.
Biochem Biophys Res Commun. 1978 Sep 14;84(1):7-14 PMID: 728136
Studies on lectins. XXXVI. Properties of some lectins prepared by affinity chromatography on O-glycosyl polyacrylamide gels.
Biochim Biophys Acta. 1978 Jan 18;538(2):299-15 PMID: 563738
Carbohydrate binding activity of a lectin-like glycoprotein from stems and leaves of Dolichos biflorus.
Biochem Biophys Res Commun. 1980 Sep 16;96(1):92-7 PMID: 7192093
Studies on lectins. XXXVIII. Isolation and characterization of the lectin from black locust bark (Robinia pseudacacia L.).
Biochim Biophys Acta. 1978 Jan 25;532(1):98-104 PMID: 620060
[Evolution of phytohemagglutinins from different parts of the seed in pea seedlings].
C R Acad Hebd Seances Acad Sci D. 1975 May 12;280(18):2105-8 PMID: 807394
The isolation and characterization of a root lectin from soybean (Glycine max (L), cultivar Chippewa).
J Biol Chem. 1981 Dec 25;256(24):12905-10 PMID: 7198118
Role of Lectins in Plant-Microorganism Interactions: II. Distribution of Soybean Lectin in Tissues of Glycine max (L.) Merr.
Plant Physiol. 1978 May;61(5):779-84 PMID: 16660384
Phytohemagglutinin isolectin subunit composition.
Biochim Biophys Acta. 1981 Mar 27;668(1):132-40 PMID: 7236703
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
Biological and biochemical properties of Phaseolus vulgaris isolectins.
J Biol Chem. 1977 May 10;252(9):2961-6 PMID: 853039
Immunization, isolation of immunoglobulins, estimation of antibody titre.
Scand J Immunol Suppl. 1973;1:161-4 PMID: 4204108
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.
J Biol Chem. 1969 Aug 25;244(16):4406-12 PMID: 5806584
Development and Distribution of Dolichos biflorus Lectin as Measured by Radioimmunoassay.
Plant Physiol. 1978 May;61(5):847-50 PMID: 16660399
Isolation and characterization of a protein from leaves and stems of Dolichos biflorus that cross reacts with antibodies to the seed lectin.
Biochemistry. 1978 Apr 18;17(8):1474-9 PMID: 646995
Distribution of lectins in membranes of soybean and peanut plants : I. General distribution in root, shoot and leaf tissue at different stages of growth.
Planta. 1979 Jan;145(2):193-8 PMID: 24317676
Agarose gel electrophoresis.
Scand J Clin Lab Invest Suppl. 1972;124:7-19 PMID: 4114361
Molecular weight determination of protein-dodecyl sulfate complexes by gel electrophoresis in a discontinuous buffer system.
J Biol Chem. 1971 Oct 25;246(20):6328-34 PMID: 5127429
Lectins and the soybean-Rhizobium symbiosis. I. Immunological investigations of soybean lines, the seeds of which have been reported to lack the 120 000 dalton soybean lectin.
Biochim Biophys Acta. 1980 May 7;629(2):292-304 PMID: 7190028
Studies on the appearance and location of hemagglutinins from a common lentil during the life cycle of the plant.
Arch Biochem Biophys. 1972 Mar;149(1):323-6 PMID: 4622779
Minimization of variation in the response to different proteins of the Coomassie blue G dye-binding assay for protein.
Anal Biochem. 1981 Sep 1;116(1):53-64 PMID: 7304986
In vitro method for testing the toxin-producing capacity of diphtheria bacteria.
Acta Pathol Microbiol Scand. 1948;25(1-2):186-91 PMID: 18887479
Purification and characterization of two lectins from Aloe arborescens Mill.
J Biochem. 1979 Jan;85(1):163-71 PMID: 104986
Purification of the phytohemagglutinin family of proteins from red kidney beans (Phaseolus vulgaris) by affinity chromatography.
Biochim Biophys Acta. 1975 Sep 9;405(1):72-81 PMID: 1174570
Studies on lectins. XLIII. Isolation and characterization of the lectin from restharrow roots (Ononis hircina Jacq.).
Biochim Biophys Acta. 1978 Mar 20;539(3):287-93 PMID: 630003
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713

Article Info

Journal

Planta

Abbr.

Planta

ISSN

0032-0935

DOI

10.1007/BF00982916

Published

1984-05-00

Pages

223-8

Language

English

Region

Germany

NLM ID

1250576

External Links

PubMed source DOI: 10.1007/BF00982916 View journal details

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Detection and characterization of a lectin from non-seed tissue ofPhaseolus vulgaris.

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