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PMID: 2423529 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Probing the structure of cytoplasm.

The Journal of cell biology ·Vol. 102 ·No. 6 ·1986-06-00 ·Pages 2015-22

Luby-Phelps K, Taylor DL, Lanni F

Abstract

We have used size-fractionated, fluorescent dextrans to probe the structure of the cytoplasmic ground substance of living Swiss 3T3 cells by fluorescence recovery after photobleaching and video image processing. The data indicate that the cytoplasm of living cells has a fluid phase viscosity four times greater than water and contains structural barriers that restrict free diffusion of dissolved macromolecules in a size-dependent manner. Assuming these structural barriers comprise a filamentous meshwork, the combined fluorescence recovery after photobleaching and imaging data suggest that the average pore size of the meshwork is in the range of 300 to 400 A, but may be as small as 200 A in some cytoplasmic domains.

MeSH Terms
Animals Cell Line Cytoplasm/analysis Dextrans Fibroblasts/analysis,cytology Fluorescein-5-isothiocyanate/analogs & derivatives Fluoresceins Macromolecular Substances Mice Microscopy, Fluorescence/methods Viscosity
Chemicals
Dextrans Fluoresceins Macromolecular Substances fluorescein isothiocyanate dextran Fluorescein-5-isothiocyanate
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Luby-Phelps K
Taylor D L
Lanni F
References (38)
38 references, click to expand
  1. Behavior of a fluorescent analogue of calmodulin in living 3T3 cells.
    J Cell Biol. 1985 Oct;101(4):1245-56 PMID: 4044638
  2. Purification of the 300K intermediate filament-associated protein and its in vitro recombination with intermediate filaments.
    J Cell Biol. 1985 Sep;101(3):802-13 PMID: 3897249
  3. Passage of dextran molecules across the blood-lymph barrier.
    Acta Chir Scand Suppl. 1956;211:1-84 PMID: 13326154
  4. Theory of diffusion in gels.
    Biophys J. 1961 Jan;1:205-13 PMID: 13759434
  5. ON THE INTERACTION BETWEEN POLYSACCHARIDES AND OTHER MACROMOLECULES. II. THE TRANSPORT OF GLOBULAR PARTICLES THROUGH HYALURONIC ACID SOLUTIONS.
    Biochim Biophys Acta. 1963 Oct 29;78:351-9 PMID: 14099644
  6. Studies on the length of the side chains of the dextran elaborated by Leuconostoc mesenteroides NRRL B-512.
    Carbohydr Res. 1971 Nov;20(1):39-48 PMID: 5151196
  7. Studies on the mechanism by which cyanine dyes measure membrane potential in red blood cells and phosphatidylcholine vesicles.
    Biochemistry. 1974 Jul 30;13(16):3315-30 PMID: 4842277
  8. The actin content of fibroblasts.
    Biochem J. 1975 May;147(2):221-8 PMID: 1180890
  9. Selective assay of monomeric and filamentous actin in cell extracts, using inhibition of deoxyribonuclease I.
    Cell. 1978 Nov;15(3):935-43 PMID: 728995
  10. Cytoplasmic structure and contractility in amoeboid cells.
    Int Rev Cytol. 1979;56:57-144 PMID: 37189
  11. The periodic association of MAP2 with brain microtubules in vitro.
    J Cell Biol. 1979 Feb;80(2):266-76 PMID: 457745
  12. Microtrabecular lattice of the cytoplasmic ground substance. Artifact or reality.
    J Cell Biol. 1979 Jul;82(1):114-39 PMID: 479294
  13. Analysis of cell surface interactions by measurements of lateral mobility.
    J Supramol Struct. 1979;12(4):481-9 PMID: 398911
  14. Filament organization revealed in platinum replicas of freeze-dried cytoskeletons.
    J Cell Biol. 1980 Jul;86(1):212-34 PMID: 6893451
  15. Preparation and characterization of a new molecular cytochemical probe: 5-iodoacetamidofluorescein-labeled actin.
    J Histochem Cytochem. 1980 Nov;28(11):1198-206 PMID: 6107318
  16. Structural interaction of cytoskeletal components.
    J Cell Biol. 1981 Jul;90(1):222-35 PMID: 7019221
  17. Association dynamics and lateral transport in biological membranes.
    J Supramol Struct Cell Biochem. 1981;17(1):61-7 PMID: 7321054
  18. Cytoplasmic and nuclear architecture in cells and tissue: form, functions, and mode of assembly.
    Cold Spring Harb Symp Quant Biol. 1982;46 Pt 2:1013-28 PMID: 6955094
  19. Mobility of cytoplasmic and membrane-associated actin in living cells.
    Proc Natl Acad Sci U S A. 1982 Aug;79(15):4660-4 PMID: 6956883
  20. Lateral mobility in membranes as detected by fluorescence recovery after photobleaching.
    Biophys J. 1982 Oct;40(1):69-75 PMID: 7139035
  21. How crowded is the cytoplasm?
    Cell. 1982 Sep;30(2):345-7 PMID: 6754085
  22. Cytoplasmic structure and contractility: the solation--contraction coupling hypothesis.
    Philos Trans R Soc Lond B Biol Sci. 1982 Nov 4;299(1095):185-97 PMID: 6129655
  23. The structure of cortical cytoplasm.
    Philos Trans R Soc Lond B Biol Sci. 1982 Nov 4;299(1095):275-89 PMID: 6129662
  24. Mobility of microinjected rhodamine actin within living chicken gizzard cells determined by fluorescence photobleaching recovery.
    Cell. 1982 Jul;29(3):835-45 PMID: 6891291
  25. Rotational diffusion of TEMPONE in the cytoplasm of Chinese hamster lung cells.
    Biophys J. 1983 Dec;44(3):405-12 PMID: 6318842
  26. Molecular architecture of the cytoplasmic matrix.
    Kroc Found Ser. 1984;16:75-86 PMID: 6201594
  27. Rheological properties of living cytoplasm: a preliminary investigation of squid axoplasm (Loligo pealei).
    Cell Motil. 1984;4(1):7-23 PMID: 6202416
  28. Diffusion of a small molecule in the cytoplasm of mammalian cells.
    Proc Natl Acad Sci U S A. 1984 Jun;81(11):3414-8 PMID: 6328515
  29. Detection and characterization of actin monomers, oligomers, and filaments in solution by measurement of fluorescence photobleaching recovery.
    Biophys J. 1984 Jul;46(1):97-110 PMID: 6743762
  30. The cytomatrix: a short history of its study.
    J Cell Biol. 1984 Jul;99(1 Pt 2):3s-12s PMID: 6086668
  31. Interaction of actin filaments with microtubules.
    J Cell Biol. 1984 Jul;99(1 Pt 2):33s-37s PMID: 6430911
  32. Nucleo-cytoplasmic flux and intracellular mobility in single hepatocytes measured by fluorescence microphotolysis.
    EMBO J. 1984 Aug;3(8):1831-6 PMID: 6207019
  33. The translational mobility of substances within the cytoplasmic matrix.
    Proc Natl Acad Sci U S A. 1984 Nov;81(21):6747-51 PMID: 6387711
  34. Diffusion of bovine serum albumin in a neutral polymer solution.
    Biopolymers. 1985 Feb;24(2):379-86 PMID: 3978223
  35. The cytoplasmic filament system in critical point-dried whole mounts and plastic-embedded sections.
    J Cell Biol. 1985 May;100(5):1474-87 PMID: 4039327
  36. Cytoplasmic motions, rheology, and structure probed by a novel magnetic particle method.
    J Cell Biol. 1985 Jul;101(1):130-40 PMID: 4040136
  37. The cytoplasmic matrix: its volume and surface area and the diffusion of molecules through it.
    Proc Natl Acad Sci U S A. 1985 Aug;82(15):5030-4 PMID: 3860842
  38. Molecular configuration of dextrans in aqueous solution.
    Nature. 1953 Jan 31;171(4344):221-2 PMID: 13036835
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.102.6.2015
Published
1986-06-00
Pages
2015-22
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2114258
Subset
IM
Grants
NIADDK NIH HHS · AM18111 · United States
NIGMS NIH HHS · GM34639 · United States
External Links
PubMed source DOI: 10.1083/jcb.102.6.2015 PMC Full Text View journal details
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