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PMID: 24224016 Published · epublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

A RhoC biosensor reveals differences in the activation kinetics of RhoA and RhoC in migrating cells.

PloS one ·Vol. 8 ·No. 11 ·2013-00-00 ·Pages e79877

Zawistowski JS, Sabouri-Ghomi M, Danuser G, Hahn KM, Hodgson L

Abstract

RhoA and RhoC GTPases share 92% amino acid sequence identity, yet play different roles in regulating cell motility and morphology. To understand these differences, we developed and validated a biosensor of RhoC activation (RhoC FLARE). This was used together with a RhoA biosensor to compare the spatio-temporal dynamics of RhoA and RhoC activity during cell protrusion/retraction and macropinocytosis. Both GTPases were activated similarly at the cell edge, but in regions more distal from the edge RhoC showed higher activation during protrusion. The two isoforms differed markedly in the kinetics of activation. RhoC was activated concomitantly with RhoA at the cell edge, but distally, RhoC activation preceded RhoA activation, occurring before edge protrusion. During macropinocytosis, differences were observed during vesicle closure and in the area surrounding vesicle formation.

MeSH Terms
Animals Biosensing Techniques/methods Cell Line Humans Kinetics Mice rhoA GTP-Binding Protein/metabolism rhoB GTP-Binding Protein/metabolism
Chemicals
rhoA GTP-Binding Protein rhoB GTP-Binding Protein
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Zawistowski Jon S
Department of Pharmacology and Lineberger Cancer Center, University of North Carolina School of Medicine, Chapel Hill, North Carolina, United States of America.
Sabouri-Ghomi Mohsen
Danuser Gaudenz
Hahn Klaus M
Hodgson Louis
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Article Info
Journal
PloS one
Abbr.
PLoS One
ISSN
1932-6203
DOI
10.1371/journal.pone.0079877
Published
2013-00-00
Epub
2013-00-05
Pages
e79877
Language
English
Region
United States
NLM ID
101285081
PMCID
PMC3818223
Subset
IM
Grants
NIGMS NIH HHS · R01 GM090317 · United States
NIGMS NIH HHS · R01 GM093121 · United States
External Links
PubMed source DOI: 10.1371/journal.pone.0079877 PMC Full Text View journal details
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