Development and chromosomal localization of genome-specific markers by polymerase chain reaction in Brassica.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik ·Vol. 82 ·No. 5 ·1991-10-00 ·Pages 627-32

Quiros CF, Hu J, This P, Chevre AM, Delseny M

Abstract

This paper reports the application of the RAPD (random amplification of polymorphic DNA sequence) markers in Brassica genetics. Forty-seven arbitrary decamer oligonucletides were used as primers to amplify genomic DNA by polymerase chain reaction. Some of the amplified products were genome specific and could be found in both diploid and derived amphidiploid species. Of a total of 65 such markers, 16 were A genome, 37 B genome, and 12 C genome specific. Of the 37 B-genome-specific markers, 11 were mapped on four independent chromosomes of B. nigra with the aid of existing B. napus-nigra disomic alien addition lines.

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Quiros C F

Department of Vegetable Crops, University of California, 95616, Davis, CA, USA.

Hu J

This P

Chevre A M

Delseny M

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10 references, click to expand

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Article Info

Journal

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik

Abbr.

Theor Appl Genet

ISSN

0040-5752

DOI

10.1007/BF00226801

Published

1991-10-00

Pages

627-32

Language

English

Region

Germany

NLM ID

0145600

External Links

PubMed source DOI: 10.1007/BF00226801 View journal details

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Development and chromosomal localization of genome-specific markers by polymerase chain reaction in Brassica.

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