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PMID: 2420323 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A comparison of rates of protein turnover in rat diaphragm in vivo and in vitro.

The Biochemical journal ·Vol. 233 ·No. 1 ·1986-01-01 ·Pages 279-82

Preedy VR, Smith DM, Sugden PH

Abstract

Protein synthesis and degradation rates in diaphragms from fed or starved rats were compared in vivo and in vitro. For fed rats, synthesis rates in vivo were approximately twice those in vitro, but for starved rats rates were similar. Degradation rates were less in vivo than in vitro in diaphragms from either fed or starved rats.

MeSH Terms
Animals Diaphragm/metabolism In Vitro Techniques Male Muscle Proteins/metabolism Organ Size RNA/metabolism Rats
Chemicals
Muscle Proteins RNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Preedy V R
Smith D M
Sugden P H
References (26)
26 references, click to expand
  1. The influence of immobilization and stretch on protein turnover of rat skeletal muscle.
    J Physiol. 1977 Jan;264(1):267-82 PMID: 839454
  2. In vitro preparations of the diaphragm and other skeletal muscles.
    Methods Enzymol. 1975;39:82-94 PMID: 1152687
  3. The influence of passive stretch on the growth and protein turnover of the denervated extensor digitorum longus muscle.
    Biochem J. 1978 Aug 15;174(2):595-602 PMID: 708412
  4. Turnover of muscle protein in the fowl. Changes in rates of protein synthesis and breakdown during hypertrophy of the anterior and posterior latissimus dorsi muscles.
    Biochem J. 1978 Nov 15;176(2):407-17 PMID: 743249
  5. The effects of cutting or of stretching skeletal muscle in vitro on the rates of protein synthesis and degradation.
    Biochem J. 1980 Apr 15;188(1):247-54 PMID: 7406883
  6. Effects of diabetes on protein turnover in cardiac muscle.
    Am J Physiol. 1980 Sep;239(3):E178-85 PMID: 7001914
  7. The effects of calcium ions, ionophore A23187 and inhibition of energy metabolism on protein degradation in the rat diaphragm and epitrochlearis muscles in vitro.
    Biochem J. 1980 Sep 15;190(3):593-603 PMID: 6781483
  8. A rapid and convenient technique for measuring the rate of protein synthesis in tissues by injection of [3H]phenylalanine.
    Biochem J. 1980 Nov 15;192(2):719-23 PMID: 6786283
  9. The effect of intermittent changes in tension on protein and collagen synthesis in isolated rabbit muscles.
    Biochem J. 1981 Sep 15;198(3):491-8 PMID: 7326016
  10. Does leucine, leucyl-tRNA, or some metabolite of leucine regulate protein synthesis and degradation in skeletal and cardiac muscle?
    J Biol Chem. 1982 Feb 25;257(4):1613-21 PMID: 6915936
  11. Arachidonic acid, prostaglandin E2 and F2 alpha influence rates of protein turnover in skeletal and cardiac muscle.
    J Biol Chem. 1982 Feb 25;257(4):1632-8 PMID: 6799511
  12. Effects of glucose, pyruvate, lactate, and amino acids on muscle protein synthesis.
    Am J Physiol. 1982 Mar;242(3):E184-92 PMID: 7065176
  13. The measurement of protein synthesis in biological systems.
    Life Sci. 1982 May 17;30(20):1679-90 PMID: 7047965
  14. The effects of glucose, acetate, lactate and insulin on protein degradation in the perfused rat heart.
    Biochem J. 1982 Sep 15;206(3):467-72 PMID: 6756391
  15. Protein turnover measured in vivo and in vitro in muscles undergoing compensatory growth and subsequent denervation atrophy.
    Biochem J. 1983 Jan 15;210(1):89-98 PMID: 6189483
  16. Effects of insulin in vitro on protein turnover in rat epitrochlearis muscle.
    Biochem J. 1983 Feb 15;210(2):323-30 PMID: 6344864
  17. Protein synthesis in skeletal muscle of the perfused rat hemicorpus compared with rates in the intact animal.
    Biochem J. 1983 Aug 15;214(2):433-42 PMID: 6193782
  18. Differential rates of protein synthesis in vitro and RNA contents in rat heart ventricular and atrial muscle.
    Biochem J. 1983 Aug 15;214(2):497-502 PMID: 6193783
  19. Stimulation of left-atrial protein-synthesis rates by increased left-atrial filling pressures in the perfused working rat heart in vitro.
    Biochem J. 1983 Dec 15;216(3):537-42 PMID: 6199013
  20. Rates of protein turnover in vivo and in vitro in ventricular muscle of hearts from fed and starved rats.
    Biochem J. 1984 Sep 1;222(2):395-400 PMID: 6206849
  21. Regional variation and differential sensitivity of rat heart protein synthesis in vivo and in vitro.
    Biochem J. 1985 Jan 15;225(2):487-92 PMID: 2579639
  22. The effect of insulin and intermittent mechanical stretching on rates of protein synthesis and degradation in isolated rabbit muscle.
    Biochem J. 1985 Aug 15;230(1):117-23 PMID: 3902005
  23. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  24. An ultrasensitive isotope dilution method for the determination of L-amino acids.
    Anal Biochem. 1970 Feb;33(2):244-54 PMID: 4909224
  25. Effects of insulin, glucose, and amino acids on protein turnover in rat diaphragm.
    J Biol Chem. 1975 Jan 10;250(1):290-8 PMID: 1141208
  26. Measurement of the rate of protein synthesis and compartmentation of heart phenylalanine.
    J Biol Chem. 1978 Feb 25;253(4):1030-40 PMID: 624716
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2330279
Published
1986-01-01
Pages
279-82
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1153017
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2330279 PMC Full Text View journal details
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