Home LiteratureArticle Details
PMID: 2419894 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A monoclonal antibody that cross-reacts with phosphorylated epitopes on two microtubule-associated proteins and two neurofilament polypeptides.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 4 ·1986-02-00 ·Pages 1006-10

Luca FC, Bloom GS, Vallee RB

Abstract

A monoclonal antibody is described that was raised against bovine brain microtubule-associated protein (MAP) 1B. Immunoblot analysis revealed that immunoreactivity was abolished by dephosphorylation of the antigen. The antigen/antibody reaction was also directly inhibited by sodium phosphate. In whole brain tissue, MAP 1B was the primary immunoreactive species. However, the antibody was also found to react with MAP 1A as well as with the high and middle molecular weight neurofilament polypeptides. No cross-reaction with MAP 2, which is known to be extensively phosphorylated, other MAPs, or the low molecular weight neurofilament polypeptide was observed. This evidence suggests at least some sequence homology between these different polypeptide components of the neuronal cytoskeleton and points to a common mechanism for their phosphorylation.

MeSH Terms
Animals Antibodies, Monoclonal/immunology Antigen-Antibody Reactions/drug effects Brain Chemistry Cattle Cross Reactions Epitopes/immunology Intermediate Filament Proteins/immunology Microtubule-Associated Proteins/immunology Neurofilament Proteins Phosphates/pharmacology Phosphoproteins/immunology Phosphorylation
Chemicals
Antibodies, Monoclonal Epitopes Intermediate Filament Proteins Microtubule-Associated Proteins Neurofilament Proteins Phosphates Phosphoproteins sodium phosphate
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Luca F C
Bloom G S
Vallee R B
References (38)
38 references, click to expand
  1. Developmental changes in components of chick brain microtubule-associated protein-1 (MAP-1) and tau proteins.
    J Biochem. 1985 Feb;97(2):529-32 PMID: 3924904
  2. Circulating autoantibodies to the 200,000-dalton protein of neurofilaments in the serum of healthy individuals.
    Science. 1985 May 31;228(4703):1117-9 PMID: 4039466
  3. Microtubule-associated protein 1B: identification of a major component of the neuronal cytoskeleton.
    Proc Natl Acad Sci U S A. 1985 Aug;82(16):5404-8 PMID: 3895231
  4. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  5. Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane.
    Biochemistry. 1971 Jun 22;10(13):2606-17 PMID: 4326772
  6. Rat brain microtubule protein: purification and determination of covalently bound phosphate and carbohydrate.
    Proc Natl Acad Sci U S A. 1972 Aug;69(8):2283-7 PMID: 4506098
  7. Cyclic AMP-dependent endogenous phosphorylation of a microtubule-associated protein.
    Proc Natl Acad Sci U S A. 1975 Jan;72(1):177-81 PMID: 164013
  8. Microtubule-associated proteins and the stimulation of tubulin assembly in vitro.
    Biochemistry. 1976 Oct 5;15(20):4497-505 PMID: 974072
  9. Removal of the projections from cytoplasmic microtubules in vitro by digestion with trypsin.
    J Biol Chem. 1977 Jan 10;252(1):377-82 PMID: 833126
  10. The periodic association of MAP2 with brain microtubules in vitro.
    J Cell Biol. 1979 Feb;80(2):266-76 PMID: 457745
  11. Multiple phosphorylation sites of microtubule-associated protein (MAP2) observed at high ATP concentrations.
    FEBS Lett. 1981 Jan 26;123(2):181-5 PMID: 7227509
  12. Study of the 10-nm-filament fraction isolated during the standard microtubule preparation.
    Biochem J. 1980 Nov 1;191(2):543-6 PMID: 7195199
  13. Phosphotyrosine-containing proteins isolated by affinity chromatography with antibodies to a synthetic hapten.
    Nature. 1981 Dec 17;294(5842):654-6 PMID: 6171737
  14. A taxol-dependent procedure for the isolation of microtubules and microtubule-associated proteins (MAPs).
    J Cell Biol. 1982 Feb;92(2):435-42 PMID: 6120944
  15. Silver staining of proteins in polyacrylamide gels.
    Anal Biochem. 1981 Nov 15;118(1):197-203 PMID: 6175245
  16. Multiple phosphorylation sites in mammalian neurofilament polypeptides.
    J Biol Chem. 1982 Sep 10;257(17):10467-70 PMID: 7202005
  17. Phosphate content of mammalian neurofilaments.
    J Biol Chem. 1982 Sep 10;257(17):9902-5 PMID: 7202006
  18. Simultaneous separation and purification of neurofilament and glial filament proteins from brain.
    J Neurochem. 1982 Jan;38(1):142-50 PMID: 6809896
  19. Serum antibodies that distinguish between the phospho- and dephospho-forms of a phosphoprotein.
    Nature. 1982 Oct 21;299(5885):734-6 PMID: 6289133
  20. Regulation of a high molecular weight microtubule-associated protein in PC12 cells by nerve growth factor.
    J Cell Biol. 1983 Jan;96(1):76-83 PMID: 6186675
  21. Phosphorylation of microtubule-associated proteins regulates their interaction with actin filaments.
    J Biol Chem. 1983 Jun 10;258(11):7064-71 PMID: 6304075
  22. Association of microtubule-associated protein 2 (MAP 2) with microtubules and intermediate filaments in cultured brain cells.
    J Cell Biol. 1983 Jun;96(6):1523-31 PMID: 6343400
  23. Monoclonal antibodies to mitotic cells.
    Proc Natl Acad Sci U S A. 1983 May;80(10):2926-30 PMID: 6574461
  24. Extensive cAMP-dependent and cAMP-independent phosphorylation of microtubule-associated protein 2.
    J Biol Chem. 1983 Jun 25;258(12):7883-6 PMID: 6305960
  25. Monoclonal antibodies distinguish phosphorylated and nonphosphorylated forms of neurofilaments in situ.
    Proc Natl Acad Sci U S A. 1983 Oct;80(19):6126-30 PMID: 6577472
  26. Characterization and use of monoclonal antibodies for isolation of phosphotyrosyl proteins from retrovirus-transformed cells and growth factor-stimulated cells.
    Mol Cell Biol. 1983 Aug;3(8):1343-52 PMID: 6194425
  27. Widespread distribution of the major polypeptide component of MAP 1 (microtubule-associated protein 1) in the nervous system.
    J Cell Biol. 1984 Jan;98(1):320-30 PMID: 6368569
  28. Widespread cellular distribution of MAP-1A (microtubule-associated protein 1A) in the mitotic spindle and on interphase microtubules.
    J Cell Biol. 1984 Jan;98(1):331-40 PMID: 6142895
  29. Ontogeny of microtubule-associated protein 2 in rat cerebellum: differential expression of the doublet polypeptides.
    Neuroscience. 1984 Jan;11(1):156-67 PMID: 6709184
  30. Phosphoproteins are components of mitotic microtubule organizing centers.
    Proc Natl Acad Sci U S A. 1984 Jul;81(14):4439-43 PMID: 6379644
  31. Use of an antiserum against phosphotyrosine for the identification of phosphorylated components in human fibroblasts stimulated by platelet-derived growth factor.
    J Biol Chem. 1984 Sep 10;259(17):11145-52 PMID: 6206062
  32. Heterogeneity of microtubule-associated protein 2 during rat brain development.
    Proc Natl Acad Sci U S A. 1984 Sep;81(17):5613-7 PMID: 6591209
  33. Microtubule-associated protein 2: monoclonal antibodies demonstrate the selective incorporation of certain epitopes into Alzheimer neurofibrillary tangles.
    Proc Natl Acad Sci U S A. 1984 Dec;81(24):7941-5 PMID: 6083566
  34. Effect upon mitogenic stimulation of calcium-dependent phosphorylation of cytoskeleton-associated 350,000- and 80,000-mol-wt polypeptides in quiescent 3Y1 cells.
    J Cell Biol. 1985 Mar;100(3):748-53 PMID: 3972893
  35. A polymer-dependent increase in phosphorylation of beta-tubulin accompanies differentiation of a mouse neuroblastoma cell line.
    J Cell Biol. 1985 Mar;100(3):764-74 PMID: 2857724
  36. Microtubule-associated proteins (MAPs): a monoclonal antibody to MAP 1 decorates microtubules in vitro but stains stress fibers and not microtubules in vivo.
    Proc Natl Acad Sci U S A. 1985 Mar;82(5):1434-8 PMID: 3883359
  37. Microheterogeneity of microtubule-associated proteins, MAP-1 and MAP-2, and differential phosphorylation of individual subcomponents.
    J Biol Chem. 1985 May 10;260(9):5797-803 PMID: 2985613
  38. Cytoskeletal architecture and immunocytochemical localization of microtubule-associated proteins in regions of axons associated with rapid axonal transport: the beta,beta'-iminodipropionitrile-intoxicated axon as a model system.
    J Cell Biol. 1985 Jul;101(1):227-39 PMID: 2409096
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.4.1006
Published
1986-02-00
Pages
1006-10
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC322999
Subset
IM
Grants
NIGMS NIH HHS · GM26701 · United States
External Links
PubMed source DOI: 10.1073/pnas.83.4.1006 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2