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PMID: 2419485 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Review

Calcium-gated calcium channels in sarcoplasmic reticulum of rabbit skinned skeletal muscle fibers.

The Journal of general physiology ·Vol. 87 ·No. 2 ·1986-02-00 ·Pages 289-303

Volpe P, Salviati G, Chu A

Abstract

The action of ruthenium red (RR) on Ca2+ loading by and Ca2+ release from the sarcoplasmic reticulum (SR) of chemically skinned skeletal muscle fibers of the rabbit was investigated. Ca2+ loading, in the presence of the precipitating anion pyrophosphate, was monitored by a light-scattering method. Ca2+ release was indirectly measured by following tension development evoked by caffeine. Stimulation of the Ca2+ loading rate by 5 microM RR was dependent on free Ca2+, being maximal at pCa 5.56. Isometric force development induced by 5 mM caffeine was reversibly antagonized by RR. IC50 for the rate of tension rise was 0.5 microM; that for the extent of tension was 4 microM. RR slightly shifted the steady state isometric force/pCa curve toward lower pCa values. At 5 microM RR, the pCa required for half-maximal force was 0.2 log units lower than that of the control, and maximal force was depressed by approximately 16%. These results suggest that RR inhibited Ca2+ release from the SR and stimulated Ca2+ loading into the SR by closing Ca2+-gated Ca2+ channels. Previous studies on isolated SR have indicated the selective presence of such channels in junctional terminal cisternae.

MeSH Terms
Animals Biological Transport, Active/drug effects Caffeine/pharmacology Calcium/metabolism In Vitro Techniques Ion Channels/drug effects,metabolism Muscle Contraction/drug effects Muscles/drug effects,physiology Rabbits Ruthenium Red/pharmacology Sarcoplasmic Reticulum/drug effects,metabolism
Chemicals
Ion Channels Ruthenium Red Caffeine Calcium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Volpe P
Salviati G
Chu A
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40 references, click to expand
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Article Info
Journal
The Journal of general physiology
Abbr.
J Gen Physiol
ISSN
0022-1295
DOI
10.1085/jgp.87.2.289
Published
1986-02-00
Pages
289-303
Language
English
Region
United States
NLM ID
2985110R
PMCID
PMC2217604
Subset
IM
External Links
PubMed source DOI: 10.1085/jgp.87.2.289 PMC Full Text View journal details
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