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PMID: 24185898 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Autophagy proteins control goblet cell function by potentiating reactive oxygen species production.

The EMBO journal ·Vol. 32 ·No. 24 ·2013-12-11 ·Pages 3130-44

Patel KK, Miyoshi H, Beatty WL, Head RD, Malvin NP, Cadwell K, Guan JL, Saitoh T, Akira S, Seglen PO, Dinauer MC, Virgin HW, Stappenbeck TS

Abstract

Delivery of granule contents to epithelial surfaces by secretory cells is a critical physiologic process. In the intestine, goblet cells secrete mucus that is required for homeostasis. Autophagy proteins are required for secretion in some cases, though the mechanism and cell biological basis for this requirement remain unknown. We found that in colonic goblet cells, proteins involved in initiation and elongation of autophagosomes were required for efficient mucus secretion. The autophagy protein LC3 localized to intracellular multi-vesicular vacuoles that were consistent with a fusion of autophagosomes and endosomes. Using cultured intestinal epithelial cells, we found that NADPH oxidases localized to and enhanced the formation of these LC3-positive vacuoles. Both autophagy proteins and endosome formation were required for maximal production of reactive oxygen species (ROS) derived from NADPH oxidases. Importantly, generation of ROS was critical to control mucin granule accumulation in colonic goblet cells. Thus, autophagy proteins can control secretory function through ROS, which is in part generated by LC3-positive vacuole-associated NADPH oxidases. These findings provide a novel mechanism by which autophagy proteins can control secretion.

MeSH Terms
Animals Autophagy Autophagy-Related Protein 5 Cells, Cultured Colon/cytology Endocytosis Epithelial Cells/metabolism Goblet Cells/cytology,metabolism,physiology Mice Mice, Mutant Strains Microtubule-Associated Proteins/genetics,metabolism Mucins/metabolism Mutation NADPH Oxidases/metabolism Phagosomes/metabolism Reactive Oxygen Species/metabolism Vesicular Transport Proteins/metabolism
Chemicals
Atg5 protein, mouse Autophagy-Related Protein 5 Map1lc3b protein, mouse Microtubule-Associated Proteins Mucins Reactive Oxygen Species Vesicular Transport Proteins early endosome antigen 1 NADPH Oxidases
Authors & Affiliations
13 authors, click to expand affiliations / ORCID
Patel Khushbu K
Department of Pathology and Immunology, Washington University School of Medicine, St Louis, MO, USA.
Miyoshi Hiroyuki
Beatty Wandy L
Head Richard D
Malvin Nicole P
Cadwell Ken
Guan Jun-Lin
Saitoh Tatsuya
Akira Shizuo
Seglen Per O
Dinauer Mary C
Virgin Herbert W
Stappenbeck Thaddeus S
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
1460-2075
Published
2013-12-11
Epub
2013-00-01
Pages
3130-44
Language
English
Region
England
NLM ID
8208664
PMCID
PMC3981139
Subset
IM
Grants
NIAID NIH HHS · U19 AI070489 · United States
NIAID NIH HHS · T32 AI717231 · United States
NIAID NIH HHS · R01 AI08488702 · United States
NIDDK NIH HHS · P30-DK52574 · United States
NIDDK NIH HHS · P30 DK052574 · United States
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