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PMID: 24173165 Published · ppublish English Journal Article

Determination of the driving force of the Na(+) pump in toad bladder by means of vasopressin.

The Journal of membrane biology ·Vol. 5 ·No. 4 ·1971-12-00 ·Pages 366-85

Yonath J, Civan MM

Abstract

Vasopressin stimulates Na(+) transport across toad bladder largely or entirely by decreasing the resistance to Na(+) entry into the transporting epithelial cells. Therefore, the hormone should induce proportional changes in short circuit current (I S ) and tissue conductance; the ratio of these changes should equal the driving force (E Na) of the Na(+) pump.Administration of vasopressin provided a rapid, reversible and reproducible technique for the measurement ofE Na. Values calculated forE Na ranged from 74 to 186 mV, in agreement with previously published estimates. The results were not dependent on the vasopressin concentration over a wide range of concentrations.Ouabain, an agent thought to inhibit specifically the Na(+) pump, decreased bothI S andE Na. On the other hand, amiloride, a diuretic thought to block specifically Na(+) entry, markedly reducedI S , without reducingE Na.It is concluded that vasopressin constitutes a probe for the rapid reproducible determination ofE Na under a wide variety of physiological conditions.

Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Yonath J
Laboratory of Renal Biophysics, Massachusetts General Hospital, 02114, Boston, Massachusetts.
Civan M M
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Article Info
Journal
The Journal of membrane biology
Abbr.
J Membr Biol
ISSN
0022-2631
DOI
10.1007/BF01957352
Published
1971-12-00
Pages
366-85
Language
English
Region
United States
NLM ID
0211301
External Links
PubMed source DOI: 10.1007/BF01957352 View journal details
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