Abstract
A monoclonal antibody specific for an antigenic determinant on the Bacillus subtilis sporulation-induced sigma factor sigma 29 reacted with proteins similar in size to sigma 29 in extracts of sporulating Bacillus licheniformis, Bacillus amyloliquifaciens, Bacillus cereus, Bacillus natto, and Bacillus pumilus but not in extracts prepared from vegetatively growing cultures of these bacteria. These results indicate that RNA polymerase modifications, initially described for B. subtilis, are likely to be common among sporulating Bacillus spp. and that at least some of the specific modifications that are observed in sporulating B. subtilis are conserved among members of this genus.
MeSH Terms
Antibodies, Monoclonal
Bacillus/analysis,physiology
Bacterial Proteins/analysis,immunology
DNA-Directed RNA Polymerases/metabolism
Electrophoresis, Polyacrylamide Gel
Epitopes/analysis
Hot Temperature
Sigma Factor/analysis,immunology
Spores, Bacterial
Transcription Factors/analysis
Chemicals
Antibodies, Monoclonal
Bacterial Proteins
Epitopes
Sigma Factor
Transcription Factors
DNA-Directed RNA Polymerases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Trempy J E
Haldenwang W G
References (15)
15 references, click to expand
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