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PMID: 2415352 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Demonstration of immunochemical identity between the nerve growth factor-inducible large external (NILE) glycoprotein and the cell adhesion molecule L1.

The EMBO journal ·Vol. 4 ·No. 11 ·1985-11-00 ·Pages 2765-8

Bock E, Richter-Landsberg C, Faissner A, Schachner M

Abstract

The nerve growth factor-inducible large external (NILE) glycoprotein and the neural cell adhesion molecule L1 were shown to be immunochemically identical. Immunoprecipitation with L1 and NILE antibodies of [3H]fucose-labeled material from culture supernatants and detergent extracts of NGF-treated rat PC12 pheochromocytoma cells yielded comigrating bands by SDS-PAGE. NILE antibodies reacted with immunopurified L1 antigen, but not with N-CAM and other L2 epitope-bearing glycoproteins from adult mouse brain. Finally, by sequential immunoprecipitation from detergent extracts of [35S]methionine-labeled early post-natal cerebellar cell cultures or [3H]fucose-labeled NGF-treated PC12 cells, all immunoreactivity for NILE antibody could be removed by pre-clearing with L1 antibody and vice versa.

MeSH Terms
Adrenal Gland Neoplasms/analysis Animals Antibodies Antigen-Antibody Complex Antigens, Surface/analysis Cell Adhesion Molecules Cell Line Cells, Cultured Cerebellum/analysis Epitopes/analysis Fucose/metabolism Glycoproteins/analysis Immunoassay Methionine/metabolism Mice Mice, Inbred C57BL Molecular Weight Nerve Growth Factors/pharmacology Neural Cell Adhesion Molecule L1 Pheochromocytoma/analysis Rats Sulfur Radioisotopes Tritium
Chemicals
Antibodies Antigen-Antibody Complex Antigens, Surface Cell Adhesion Molecules Epitopes Glycoproteins Nerve Growth Factors Neural Cell Adhesion Molecule L1 Sulfur Radioisotopes Tritium Fucose Methionine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Bock E
Richter-Landsberg C
Faissner A
Schachner M
References (36)
36 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1985-11-00
Pages
2765-8
Language
English
Region
England
NLM ID
8208664
PMCID
PMC554576
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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