Home LiteratureArticle Details
PMID: 2413157 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Acquisition of cytotoxic T lymphocyte-specific carbohydrate differentiation antigens.

The Journal of experimental medicine ·Vol. 162 ·No. 4 ·1985-10-01 ·Pages 1275-93

Lefrancois L, Puddington L, Machamer CE, Bevan MJ

Abstract

Cytotoxic T cell (CTL)-specific activation antigens, termed CT determinants, have been detected by monoclonal antibodies (mAb) that inhibit CTL function. At the cell surface, the CT antigens are associated with the T200 glycoproteins and two other proteins of Mr 140,000 and 85,000 and are present on a secreted protein, gp155. Periodate treatment followed by binding analysis and immunoprecipitation experiments using tunicamycin-treated cells indicated that carbohydrate is necessary for CT antigen expression. Furthermore, gp155 is secreted in the presence of tunicamycin while retaining the CT antigens, and the CT determinants are added late in T200 biosynthesis, suggesting that the CT glycans are O-linked. Finally, interleukin 2 was shown to dramatically influence the expression of the CT mAb-reactive oligosaccharides present at the CTL cell surface.

MeSH Terms
Animals Antibodies, Monoclonal/immunology Antigens, Differentiation, T-Lymphocyte Antigens, Surface/analysis,biosynthesis Carbohydrates/analysis Epitopes/analysis Glycoproteins/biosynthesis Interleukin-2/physiology Methionine/metabolism Mice Mice, Inbred C57BL Mice, Inbred DBA Molecular Weight T-Lymphocytes, Cytotoxic/immunology T-Lymphocytes, Helper-Inducer/immunology Tunicamycin/pharmacology
Chemicals
Antibodies, Monoclonal Antigens, Differentiation, T-Lymphocyte Antigens, Surface Carbohydrates Epitopes Glycoproteins Interleukin-2 Tunicamycin Methionine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Lefrancois L
Puddington L
Machamer C E
Bevan M J
References (60)
60 references, click to expand
  1. Differences in the surface proteins of mouse B and T cells.
    Proc Natl Acad Sci U S A. 1975 Jan;72(1):157-61 PMID: 47173
  2. A procedure for removing red cells and dead cells from lymphoid cell suspensions.
    J Immunol Methods. 1975 Jun;7(2-3):291-300 PMID: 167077
  3. Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.
    J Biol Chem. 1977 Feb 10;252(3):1102-6 PMID: 320200
  4. Generation of cytolytic T lymphocytes in vitro. IX. induction of secondary CTL responses in primary long-term MLC by supernatants from secondary MLC.
    J Immunol. 1978 Feb;120(2):370-7 PMID: 146052
  5. Cell surface glycoproteins of murine cytotoxic T lymphocytes. I. T 145, a new cell surface glycoprotein selectively expressed on Ly 1-2+ cytotoxic T lymphocytes.
    J Exp Med. 1978 May 1;147(5):1418-34 PMID: 306416
  6. Interspecies spleen-myeloma hybrid producing monoclonal antibodies against mouse lymphocyte surface glycoprotein, T200.
    J Exp Med. 1978 Jul 1;148(1):313-23 PMID: 78961
  7. Selective affinity fractionation of murine cytotoxic T lymphocytes (CTL). Unique lectin specific binding of the CTL associated surface glycoprotein, T 145.
    J Exp Med. 1979 Feb 1;149(2):473-84 PMID: 310865
  8. Properties and applications of monoclonal antibodies directed against determinants of the Thy-1 locus.
    J Immunol. 1979 Jun;122(6):2491-7 PMID: 87465
  9. Cytotoxic T cells: Lyt phenotype and blocking of killing activity by Lyt antisera.
    Proc Natl Acad Sci U S A. 1979 Apr;76(4):1977-81 PMID: 88050
  10. Mouse alloantibodies capable of blocking cytotoxic T-cell function. I. Relationship between the antigen reactive with blocking antibodies and the Lyt-2 locus.
    J Exp Med. 1979 Sep 19;150(3):432-44 PMID: 113478
  11. Effects of N alpha-tosyl-L-lysyl-chloromethylketone on the activity of cytotoxic T lymphocytes.
    J Immunol. 1980 Mar;124(3):1028-33 PMID: 6987305
  12. A rapid method for the separation of functional lymphoid cell populations of human and animal origin on PVP-silica (Percoll) density gradients.
    Scand J Immunol. 1979;10(6):563-73 PMID: 232310
  13. T200 cell surface glycoprotein of the mouse. Polymorphism defined by the Ly-5 system of alloantigens.
    J Exp Med. 1980 May 1;151(5):1311-6 PMID: 7373223
  14. Mode of regulation of natural killer cell activity by interferon.
    J Exp Med. 1980 Jul 1;152(1):124-37 PMID: 6156979
  15. Identification of Ly-5 and T200 antigens on identical cell surface proteins.
    J Immunol. 1980 Sep;125(3):1400-1 PMID: 6157728
  16. Presence of T 145 on cytolytic T cell lines and their lectin-resistant mutants.
    Eur J Immunol. 1980 Nov;10(11):860-8 PMID: 6970130
  17. Flow cytofluorometric analysis of the binding of Vicia villosa lectin to T lymphoblasts: lack of correlation with cytolytic function.
    J Immunol. 1981 Mar;126(3):883-6 PMID: 6450806
  18. B220: a B cell-specific member of th T200 glycoprotein family.
    Nature. 1981 Feb 19;289(5799):681-3 PMID: 6970340
  19. Cell surface glycoproteins of cytotoxic T lymphocytes induced in vivo and in vitro.
    J Immunol. 1981 Apr;126(4):1443-6 PMID: 6451646
  20. Entactin, a novel basal lamina-associated sulfated glycoprotein.
    J Biol Chem. 1981 May 25;256(10):5209-14 PMID: 6262321
  21. Does OKT3 monoclonal antibody react with an antigen-recognition structure on human T cells?
    Proc Natl Acad Sci U S A. 1981 Mar;78(3):1805-8 PMID: 6165018
  22. Surface antigens on mouse natural killer cells: use of monoclonal antibodies to inhibit or to enrich cytotoxic activity.
    J Immunol. 1981 Sep;127(3):982-6 PMID: 6790624
  23. Inhibition of specific cell-mediated cytotoxicity by monoclonal antibodies to human T cell antigens.
    Proc Natl Acad Sci U S A. 1981 Jul;78(7):4500-4 PMID: 6974866
  24. Lymphocyte function-associated antigen 1 (LFA-1): a surface antigen distinct from Lyt-2,3 that participates in T lymphocyte-mediated killing.
    Proc Natl Acad Sci U S A. 1981 Jul;78(7):4535-9 PMID: 7027264
  25. T cell growth factor receptors. Quantitation, specificity, and biological relevance.
    J Exp Med. 1981 Nov 1;154(5):1455-74 PMID: 6975347
  26. Transmembrane assembly of membrane and secretory glycoproteins.
    Arch Biochem Biophys. 1981 Oct 1;211(1):1-19 PMID: 7030209
  27. Differences in carbohydrate moieties of high molecular weight glycoproteins of human lymphocytes of T and B origins revealed by monoclonal autoantibodies with anti-I and anti-i specificities.
    Biochem Biophys Res Commun. 1981 Oct 30;102(4):1158-64 PMID: 6172121
  28. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  29. The enzymatic iodination of the red cell membrane.
    J Cell Biol. 1972 Nov;55(2):390-405 PMID: 5076780
  30. Purification and properties of an endo-beta-N-acetylglucosaminidase from Streptomyces griseus.
    J Biol Chem. 1974 Feb 10;249(3):811-7 PMID: 4204552
  31. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.
    Eur J Biochem. 1974 Jul 1;46(1):83-8 PMID: 4850204
  32. Characterization of five cloned murine cell lines showing high cytolytic activity against YAC-1 cells.
    J Immunol. 1982 May;128(5):2326-35 PMID: 6174628
  33. The specificity of the combining site of the lectin from Vicia villosa seeds which react with cytotoxic T-lymphoblasts.
    Mol Immunol. 1981 Nov;18(11):969-77 PMID: 6176850
  34. Carbohydrate moieties of glycoproteins. A re-evaluation of their function.
    Biochim Biophys Acta. 1982 May 12;650(4):209-32 PMID: 7046800
  35. Calcium facilitates endogenous proteolysis of the EGF receptor-kinase.
    Mol Cell Endocrinol. 1982 Aug;27(3):263-76 PMID: 6290288
  36. Monoclonal antibody to murine embryos defines a stage-specific embryonic antigen expressed on mouse embryos and human teratocarcinoma cells.
    Cell. 1982 Oct;30(3):697-705 PMID: 6183004
  37. Synthesis and localization of two sulphated glycoproteins associated with basement membranes and the extracellular matrix.
    J Cell Biol. 1982 Oct;95(1):197-204 PMID: 7142285
  38. Clonotypic structures involved in antigen-specific human T cell function. Relationship to the T3 molecular complex.
    J Exp Med. 1983 Feb 1;157(2):705-19 PMID: 6185617
  39. Isolation and characterization of lectins from Vicia villosa. Two distinct carbohydrate binding activities are present in seed extracts.
    J Biol Chem. 1983 Apr 25;258(8):5165-71 PMID: 6833294
  40. A clone-specific monoclonal antibody that inhibits cytolysis of a cytolytic T cell clone.
    J Exp Med. 1983 Mar 1;157(3):921-35 PMID: 6187881
  41. On the function of Ly-5 in the regulation of antigen-driven B cell differentiation. Comparison and contrast with Lyb-2.
    J Exp Med. 1983 Apr 1;157(4):1077-88 PMID: 6220106
  42. Evidence for the occurrence of O-glycosidically linked oligosaccharides of poly-N-acetyllactosamine type on the human leucocyte common antigen.
    Biochem Biophys Res Commun. 1983 Jan 27;110(2):424-31 PMID: 6601483
  43. Different forms of Ly-5 within the T-cell lineage.
    Immunogenetics. 1983;17(6):649-54 PMID: 6602762
  44. Lymphokine-driven "differentiation" of cytotoxic T-cell clones into cells with NK-like specificity: correlations with display of membrane macromolecules.
    Immunol Rev. 1983;72:43-72 PMID: 6192076
  45. Blockade of NK cell lysis is a property of monoclonal antibodies that bind to distinct regions of T-200.
    J Immunol. 1983 Oct;131(4):1742-7 PMID: 6194213
  46. Characterization of the murine T cell surface molecule, designated L3T4, identified by monoclonal antibody GK1.5: similarity of L3T4 to the human Leu-3/T4 molecule.
    J Immunol. 1983 Nov;131(5):2445-51 PMID: 6415170
  47. The site of action of N-alpha-tosyl-L-lysyl-chloromethyl-ketone (TLCK) on cloned cytotoxic T lymphocytes.
    J Immunol. 1983 Nov;131(5):2477-83 PMID: 6415172
  48. Evidence that Ly-5 product of T and B cells differ in protein structure.
    Immunogenetics. 1984;19(2):149-54 PMID: 6607883
  49. Antigen-independent activation of memory cytotoxic T cells by interleukin 2.
    J Immunol. 1984 Apr;132(4):1845-50 PMID: 6230394
  50. Inhibition of T cell responses to alloantigens and polyclonal mitogens by Ly-5 antisera.
    J Immunol. 1984 Jul;133(1):10-5 PMID: 6233370
  51. Neural cell adhesion molecules and myelin-associated glycoprotein share a common carbohydrate moiety recognized by monoclonal antibodies L2 and HNK-1.
    Nature. 1984 Sep 13-19;311(5982):153-5 PMID: 6206400
  52. Activated B cells express receptors for, and proliferate in response to, pure interleukin 2.
    J Exp Med. 1984 Oct 1;160(4):1170-83 PMID: 6434689
  53. Monoclonal antibodies against carbohydrate differentiation antigens identify subsets of primary sensory neurones.
    Nature. 1984 Oct 4-10;311(5985):469-72 PMID: 6207435
  54. A murine cytotoxic T lymphocyte cell line resistant to Vicia villosa lectin is deficient in UDP-GalNAc:beta-galactose beta 1,4-N-acetylgalactosaminyltransferase.
    J Biol Chem. 1984 Oct 25;259(20):12536-42 PMID: 6436236
  55. Blood group-active carbohydrate chains on the receptor for epidermal growth factor of A431 cells.
    EMBO J. 1984 Oct;3(10):2227-33 PMID: 6094178
  56. Co-immunoprecipitation of the Ly-5 molecule and an endogenous protease: a proteolytic system requiring a reducing agent and Ca2+1.
    J Immunol. 1985 Apr;134(4):2513-9 PMID: 3919099
  57. Functional modifications of cytotoxic T-lymphocyte T200 glycoprotein recognized by monoclonal antibodies.
    Nature. 1985 Apr 4-10;314(6010):449-52 PMID: 2580241
  58. Evidence from cDNA clones that the rat leukocyte-common antigen (T200) spans the lipid bilayer and contains a cytoplasmic domain of 80,000 Mr.
    Cell. 1985 May;41(1):83-93 PMID: 3158393
  59. Novel antigenic determinants of the T200 glycoprotein expressed preferentially by activated cytotoxic T lymphocytes.
    J Immunol. 1985 Jul;135(1):374-83 PMID: 2582040
  60. Beta-linked N-acetylgalactosamine residues present at the nonreducing termini of O-linked oligosaccharides of a cloned murine cytotoxic T lymphocyte line are absent in a Vicia villosa lectin-resistant mutant cell line.
    J Biol Chem. 1984 Oct 25;259(20):12528-35 PMID: 6333421
Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.162.4.1275
Published
1985-10-01
Pages
1275-93
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2187862
Subset
IM
Grants
NIAID NIH HHS · AI19335 · United States
NIAID NIH HHS · AI19499 · United States
External Links
PubMed source DOI: 10.1084/jem.162.4.1275 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2