Multiplex detection of DNA mutations by the fluorescence fingerprint spectrum technique.

Angewandte Chemie (International ed. in English) ·第 52 卷 ·第 49 期 ·2014-09-12

Song Jinzhao, Zhang Jiangyan, Lv Fengting, Cheng Yongqiang, Wang Bing, Feng Liheng, Liu Libing, Wang Shu

摘要

A fingerprint spectrum technique that utilizes cationic conjugated-polymer-based fluorescence resonance energy transfer (FRET) is used for multiplex detection of DNA mutations. This method detects as low as 5 % mutation of the total DNA. Ten PIK3CA mutations originating from 30 clinical breast cancer samples could be detected.

关键词

DNA mutation FRET biosensors conjugated polymers fingerprint spectrum

文献信息

期刊

Angewandte Chemie (International ed. in English)

期刊简称

Angew Chem Int Ed Engl

ISSN

1433-7851

DOI

10.1002/anie.201305461

发表日期

2014-09-12

收录日期

2013-11-27

更新日期

2013-11-27

语言

英语

国家/地区

Germany

NLM ID

0370543

外部链接

PubMed 原文 DOI: 10.1002/anie.201305461 查看期刊详情

分析服务

Multiplex detection of DNA mutations by the fluorescence fingerprint spectrum technique.

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