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PMID: 2412202 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Calcium-dependent inward currents in voltage-clamped guinea-pig olfactory cortex neurones.

Pflugers Archiv : European journal of physiology ·Vol. 404 ·No. 3 ·1985-07-00 ·Pages 259-65

Constanti A, Galvan M, Franz P, Sim JA

Abstract

Guinea-pig olfactory cortex neurones in vitro (23 degrees C--25 degrees C) were voltage clamped by means of a single microelectrode sample-and-hold technique. In most Cs+-loaded neurones (in the presence of tetrodotoxin), membrane depolarization beyond -60 mV elicited inward currents, which had rapid activation kinetics. The steady-state current-voltage relationship was N-shaped with a region of negative slope conductance between - 50 mV and - 20 mV. The rate of inactivation varied according to the holding potential and the command potential. The inward currents were maintained when external Ca2+ was replaced by Ba2+, and were blocked by Cd2+, suggesting that Ca2+ was the principal charge carrier. The results demonstrate the existence of calcium current in olfactory cortex neurones.

MeSH Terms
Animals Barium/pharmacology Cadmium/pharmacology Calcium/metabolism Central Nervous System/metabolism Cerebral Cortex/cytology,metabolism Cesium/pharmacology Electrophysiology Guinea Pigs Ion Channels/drug effects,physiology Neurons/metabolism Olfactory Pathways/cytology,metabolism Tetraethylammonium Tetraethylammonium Compounds/pharmacology
Chemicals
Ion Channels Tetraethylammonium Compounds Cadmium Cesium Barium Tetraethylammonium Calcium
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Constanti A
Galvan M
Franz P
Sim J A
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35 references, click to expand
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Article Info
Journal
Pflugers Archiv : European journal of physiology
Abbr.
Pflugers Arch
ISSN
0031-6768
DOI
10.1007/BF00581248
Published
1985-07-00
Pages
259-65
Language
English
Region
Germany
NLM ID
0154720
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00581248 View journal details
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