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题目:: Microbead-based biomimetic synthetic neighbors enhance survival and function of rat pancreatic β-cells.
作者:: Li(Wei),Lee(Samuel),Ma(Minglin),Kim(Soo Min),Guye(Patrick),Pancoast(James R),Anderson(Daniel G),Weiss(Ron),Lee(Richard T),Hammond(Paula T)
状态:: 发布时间2013-10-04 , 更新时间 2016-10-25
期刊:: Sci Rep
摘要:: Diabetes is caused by the loss or dysfunction of insulin-secreting β-cells in the pancreas. β-cells reduce their mass and lose insulin-producing ability in vitro, likely due to insufficient cell-cell and cell-extracellular matrix (ECM) interactions as β-cells lose their native microenvironment. Herein, we built an ex-vivo cell microenvironment by culturing primary β-cells in direct contact with 'synthetic neighbors', cell-sized soft polymer microbeads that were modified with cell-cell signaling factors as well as components from pancreatic-tissue-specific ECMs. This biomimetic 3D microenvironment was able to promote native cell-cell and cell-ECM interactions. We obtained sustained maintenance of β-cell function in vitro enhanced cell viability from the few days usually observed in 2D culture to periods exceeding three weeks, with enhanced β-cell stability and insulin production. Our approach can be extended to create a general 3D culture platform for other cell types.
语言:: eng
DOI:

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