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PMID: 2407607 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Mechanisms of gene conversion in Saccharomyces cerevisiae.

Genetics ·Vol. 124 ·No. 1 ·1990-01-00 ·Pages 7-25

Roman H, Ruzinski MM

Abstract

In red-white sectored colonies of Saccharomyces cerevisiae, derived from mitotic cells grown to stationary phase and irradiated with a light dose of x-rays, all of the segregational products of gene conversion and crossing over can be ascertained. Approximately 80% of convertants are induced in G1, the remaining 20% in G2. Crossing over, in the amount of 20%, is found among G1 convertants but most of the crossovers are delayed until G2. About 20% of all sectored colonies had more than one genotype in one or the other sector, thus confirming the hypothesis that conversion also occurs in G2. The principal primary event in G2 conversion is a single DNA heteroduplex. It is suggested that the close contact that this implies carries over to G2 when crossing over and a second round of conversion occurs.

MeSH Terms
Chromosomes, Fungal/metabolism Crossing Over, Genetic DNA, Fungal/genetics Diploidy Gene Conversion Genotype Heterozygote Mosaicism/genetics Nucleic Acid Heteroduplexes/genetics Saccharomyces cerevisiae/genetics,radiation effects
Chemicals
DNA, Fungal Nucleic Acid Heteroduplexes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Roman H
Department of Genetics, University of Washington, Seattle 98195.
Ruzinski M M
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/124.1.7
Published
1990-01-00
Pages
7-25
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1203911
Subset
IM
Grants
NIGMS NIH HHS · GM 27949 · United States
External Links
PubMed source DOI: 10.1093/genetics/124.1.7 PMC Full Text View journal details
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