Isolation and functional expression of pituitary peptidylglycine alpha-amidating enzyme mRNA. A variant lacking the transmembrane domain.

FEBS letters ·Vol. 269 ·No. 2 ·1990-09-03 ·Pages 319-23

Kato I, Yonekura H, Yamamoto H, Okamoto H

Abstract

We demonstrate that, in rat pituitary, peptidylglycine alpha-amidating enzyme was encoded by at least 5 distinct mRNAs. Southern blot and ribonuclease protection analyses revealed that the mRNAs arose through alternative splicing. A variant lacking the transmembrane domain-coding sequence was a major mRNA species for the enzyme in the pituitary. When the cDNAs were expressed in COS-7 cells, the variant was the most efficient in producing a secretory form (37 kDA) of the enzyme.

MeSH Terms

Amino Acid Sequence Animals Base Sequence Blotting, Southern Cell Line Cell Membrane/enzymology DNA/genetics,isolation & purification Gene Expression Regulation, Enzymologic Gene Library Genetic Variation Kinetics Mixed Function Oxygenases/genetics,metabolism Molecular Sequence Data Multienzyme Complexes Pituitary Gland/enzymology Plasmids RNA, Messenger/genetics,isolation & purification Rats Rats, Inbred Strains Restriction Mapping Transfection

Chemicals

Multienzyme Complexes RNA, Messenger DNA Mixed Function Oxygenases peptidylglycine monooxygenase

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Kato I

Department of Biochemistry, Tohoku University School of Medicine, Miyagi, Japan.

Yonekura H

Yamamoto H

Okamoto H

Article Info

Journal

FEBS letters

Abbr.

FEBS Lett

ISSN

0014-5793

DOI

10.1016/0014-5793(90)81184-p

Published

1990-09-03

Pages

319-23

Language

English

Region

England

NLM ID

0155157

Subset

Databases

GENBANK

X59685, X59686, X59687, X59688, X59689

External Links

PubMed source DOI: 10.1016/0014-5793(90)81184-p View journal details

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Isolation and functional expression of pituitary peptidylglycine alpha-amidating enzyme mRNA. A variant lacking the transmembrane domain.

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