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PMID: 2395883 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Polarized integrin mediates human keratinocyte adhesion to basal lamina.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 87 ·No. 17 ·1990-09-00 ·Pages 6888-92

De Luca M, Tamura RN, Kajiji S, Bondanza S, Rossino P, Cancedda R, Marchisio PC, Quaranta V

Abstract

Epithelial cell interactions with matrices are critical to tissue organization. Indirect immunofluorescence and immunoprecipitations of cell lysates prepared from stratified cultures of human epidermal cells showed that the major integrins expressed by keratinocytes are alpha E beta 4 (also called alpha 6 beta 4) and alpha 2 beta 1. The alpha E beta 4 integrin is localized at the surface of basal cells in contact with the basement membrane, whereas alpha 2 beta 1/alpha 3 beta 1 integrins are absent from the basal surface and are localized only on the lateral surface of basal and spinous keratinocytes. Anti-beta 4 antibodies potently inhibited keratinocyte adhesion to matrigel or purified laminin, whereas anti-beta 1 antibodies were ineffective. Only anti-beta 4 antibodies were able to detach established keratinocyte colonies. These data suggest that alpha E beta 4 mediates keratinocyte adhesion to basal lamina, whereas the beta 1 subfamily is involved in cell-cell adhesion of keratinocytes.

MeSH Terms
Animals Antibodies, Monoclonal Cell Adhesion Cells, Cultured Epithelium/physiology Humans Integrins/isolation & purification,physiology Keratinocytes/cytology,physiology Laminin Mice Reference Values
Chemicals
Antibodies, Monoclonal Integrins Laminin
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
De Luca M
Laboratorio di Differenziamento Cellulare, IST, Istituto Nazionale per la Ricerca sul Cancro, Genoa, Italy.
Tamura R N
Kajiji S
Bondanza S
Rossino P
Cancedda R
Marchisio P C
Quaranta V
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.87.17.6888
Published
1990-09-00
Pages
6888-92
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC54643
Subset
IM
Grants
NCI NIH HHS · CA47541 · United States
NCI NIH HHS · CA47858 · United States
External Links
PubMed source DOI: 10.1073/pnas.87.17.6888 PMC Full Text View journal details
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