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PMID: 2395862 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Gene activation is required for developmentally programmed cell death.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 87 ·No. 17 ·1990-09-00 ·Pages 6594-8

Schwartz LM, Kosz L, Kay BK

Abstract

The intersegmental muscles of the tobacco hawkmoth Manduca sexta die during the 36-hr period after metamorphosis. The trigger for cell death is a fall in the ecdysteroid titer. Commitment of the intersegmental muscles to degenerate involves selective repression and activation of ecdysteroid-responsive genes. When the pattern of gene expression is altered after injection of either 20-hydroxyecdysone or actinomycin D, the muscles persist. cDNA clones have been isolated for four genes that become abundantly expressed coincident with the commitment to degenerate. The data presented here indicate that programmed cell death is not due to the cessation of macromolecular synthesis in condemned cells but rather is due to the activation of a differentiative pathway.

MeSH Terms

Aging Animals Cell Survival/drug effects DNA/genetics Dactinomycin/pharmacology Ecdysterone/pharmacology Electrophoresis, Gel, Two-Dimensional Electrophoresis, Polyacrylamide Gel Gene Expression Regulation Gene Library Lepidoptera/growth & development Metamorphosis, Biological Moths/genetics,growth & development Muscle Development Muscle Proteins/biosynthesis,genetics,isolation & purification Muscles/drug effects RNA, Messenger/genetics Transcriptional Activation

Chemicals

Muscle Proteins RNA, Messenger Dactinomycin Ecdysterone DNA

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Schwartz L M

Department of Zoology, University of Massachusetts, Amherst 01003.

Kosz L

Kay B K

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25 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.87.17.6594

Published

1990-09-00

Pages

6594-8

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC54583

Subset

Grants

NIGMS NIH HHS · GM40458 · United States

External Links

PubMed source DOI: 10.1073/pnas.87.17.6594 PMC Full Text View journal details

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