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PMID: 2393702 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The membrane skeleton of erythrocytes. A percolation model.

Biophysical journal ·Vol. 57 ·No. 6 ·1990-06-00 ·Pages 1167-77

Saxton MJ

Abstract

The spectrin network on the cytoplasmic surface of the erythrocyte membrane is modeled as a triangular lattice of spectrin tetramers. This network obstructs lateral diffusion of proteins and provides mechanical reinforcement to the membrane. These effects are treated in a systematic and unified manner in terms of a percolation model. The diffusion coefficient is obtained as a function of the fraction of normal spectrin tetramers for both static and fluctuating barriers. The elasticity of the network is calculated as a function of the fraction of normal spectrin and the ratio of bending to stretching energies. For static barriers, elasticity and lateral diffusion are incompatible: if a network is connected enough to be elastic, it is connected enough to block long-range lateral diffusion. The elasticity and the force required for mechanical breakdown go to zero at the percolation threshold; experimental evidence suggests the existence of a stability threshold at or near the percolation threshold. The model is qualitatively applicable to other cells with membrane skeletons, such as epithelial cells, in which localization of membrane proteins is essential to differentiation.

MeSH Terms
Algorithms Diffusion Erythrocyte Membrane/metabolism,ultrastructure Humans Macromolecular Substances Mathematics Models, Structural Monte Carlo Method Spectrin/metabolism,ultrastructure
Chemicals
Macromolecular Substances Spectrin
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Saxton M J
Plant Growth Laboratory, University of California, Davis 95616.
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Article Info
Journal
Biophysical journal
Abbr.
Biophys J
ISSN
0006-3495
DOI
10.1016/S0006-3495(90)82636-9
Published
1990-06-00
Pages
1167-77
Language
English
Region
United States
NLM ID
0370626
PMCID
PMC1280827
Subset
IM
Grants
NIGMS NIH HHS · GM38133 · United States
External Links
PubMed source DOI: 10.1016/S0006-3495(90)82636-9 PMC Full Text View journal details
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