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PMID: 23929340 Published · ppublish English Letter Research Support, Non-U.S. Gov't

Targeted mutagenesis in the model plant Nicotiana benthamiana using Cas9 RNA-guided endonuclease.

Nature biotechnology ·Vol. 31 ·No. 8 ·2013-08-00 ·Pages 691-3

Nekrasov V, Staskawicz B, Weigel D, Jones JD, Kamoun S

Abstract

暂无摘要

MeSH Terms

Agrobacterium tumefaciens/genetics Bacterial Proteins/genetics Base Sequence CRISPR-Associated Protein 9 CRISPR-Associated Proteins/genetics CRISPR-Cas Systems/genetics Endonucleases/genetics Gene Editing/methods Gene Targeting Genome, Plant Homologous Recombination Mutagenesis, Site-Directed/methods Mutation Rate RNA, Guide/genetics Tobacco/genetics

Chemicals

Bacterial Proteins CRISPR-Associated Proteins RNA, Guide CRISPR-Associated Protein 9 Cas9 endonuclease Streptococcus pyogenes Endonucleases

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Nekrasov Vladimir

Staskawicz Brian

Weigel Detlef

Jones Jonathan D G

Kamoun Sophien

References (9)

9 references, click to expand

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Mol Plant Microbe Interact. 2000 Apr;13(4):439-46 PMID: 10755307
High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells.
Nat Biotechnol. 2013 Sep;31(9):822-6 PMID: 23792628
RNA-guided human genome engineering via Cas9.
Science. 2013 Feb 15;339(6121):823-6 PMID: 23287722
Multiplex genome engineering using CRISPR/Cas systems.
Science. 2013 Feb 15;339(6121):819-23 PMID: 23287718
Nicotiana benthamiana: its history and future as a model for plant-pathogen interactions.
Mol Plant Microbe Interact. 2008 Aug;21(8):1015-26 PMID: 18616398
Plant genome engineering with sequence-specific nucleases.
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Article Info

Journal

Nature biotechnology

Abbr.

Nat Biotechnol

ISSN

1546-1696

DOI

10.1038/nbt.2655

Published

2013-08-00

Pages

691-3

Language

English

Region

United States

NLM ID

9604648

Subset

Grants

Biotechnology and Biological Sciences Research Council · United Kingdom

External Links

PubMed source DOI: 10.1038/nbt.2655 View journal details

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Targeted mutagenesis in the model plant Nicotiana benthamiana using Cas9 RNA-guided endonuclease.

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