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PMID: 2391355 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The crosslinking of nuclear protein to DNA using ionizing radiation.

Journal of cancer research and clinical oncology ·Vol. 116 ·No. 4 ·1990-00-00 ·Pages 324-30

Cress AE, Kurath KM, Stea B, Bowden GT

Abstract

DNA-protein complexes were generated in intact Chinese hamster ovary (CHO) cells by the use of ionizing radiation. The DNA-protein crosslinks, as measured by a filter-binding assay, occurred immediately following the irradiation, were produced in a dose-dependent manner and were reversible. The reversibility of the crosslinks in the intact cells was dependent upon general protein synthesis. Three proteins that were attached to DNA in unirradiated cells were analyzed according to the presence of DNA attached to the proteins before, during and after exposure to ionizing radiation. All three proteins contained more DNA reversibly attached to the proteins after exposure to 5 Gy ionizing radiation as compared to unirradiated cells. One of the proteins was increasingly attached to DNA using 2.5-50 Gy X-ray. These data suggest that the increased DNA-protein crosslinking observed with ionizing radiation may involve the increase in particular protein(s) crosslinked to DNA as well as an increase in the amount of DNA attached to specific proteins.

MeSH Terms
Antibodies, Monoclonal Cells, Cultured DNA/analysis,metabolism,radiation effects DNA Damage Nuclear Proteins/analysis,metabolism,radiation effects Puromycin/pharmacology
Chemicals
Antibodies, Monoclonal Nuclear Proteins Puromycin DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Cress A E
Department of Radiation Oncology, University of Arizona Cancer Center, Tucson 85724.
Kurath K M
Stea B
Bowden G T
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Article Info
Journal
Journal of cancer research and clinical oncology
Abbr.
J Cancer Res Clin Oncol
ISSN
0171-5216
DOI
10.1007/BF01612913
Published
1990-00-00
Pages
324-30
Language
English
Region
Germany
NLM ID
7902060
Subset
IM
Grants
NCRR NIH HHS · 2SO7RR05675 · United States
NCI NIH HHS · CA-23074 · United States
NCI NIH HHS · CA-31010 · United States
External Links
PubMed source DOI: 10.1007/BF01612913 View journal details
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