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PMID: 239028 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Human brain in tissue culture. I. Acquisition, initial processing, and establishment of brain cell cultures.

The Journal of comparative neurology ·Vol. 161 ·No. 3 ·1975-06-01 ·Pages 295-306

Gilden DH, Devlin M, Wroblewska Z, Friedman H, Rorke LB, Santoli D, Koprowski H

Abstract

This paper details the in vitro techniques used to establish cells in culture from the brains of 40 patients, most of whom had chronic neurologic disease. The clinical and pathologic features of these patients are given. The success in establihsing cell lines was dependent upon the origin of tissue (biopsy vs. autopsy), the site of removal from the brain, and various environmental and technical manipulations in vitro.

MeSH Terms

Adolescent Adult Autopsy Axons/cytology Biopsy/adverse effects Brain/cytology,surgery Cell Adhesion/drug effects Cell Line Cells, Cultured Choroid Plexus/cytology Culture Media Female Freezing Humans Hydrogen-Ion Concentration Male Methods Middle Aged Multiple Sclerosis/pathology Nerve Fibers, Myelinated/cytology Optic Nerve/cytology Time Factors Trypsin/pharmacology

Chemicals

Culture Media Trypsin

Authors & Affiliations

7 authors, click to expand affiliations / ORCID

Gilden D H

Devlin M

Wroblewska Z

Friedman H

Rorke L B

Santoli D

Koprowski H

Article Info

Journal

The Journal of comparative neurology

Abbr.

J Comp Neurol

ISSN

0021-9967

DOI

10.1002/cne.901610302

Published

1975-06-01

Pages

295-306

Language

English

Region

United States

NLM ID

0406041

Subset

External Links

PubMed source DOI: 10.1002/cne.901610302 View journal details

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Human brain in tissue culture. I. Acquisition, initial processing, and establishment of brain cell cultures.

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