The degradation of T7 DNA in converging flow.

Nucleic acids research ·Vol. 18 ·No. 15 ·1990-08-11 ·Pages 4469-70

Zimm BH, Reese HR

Abstract

The flow-induced degradation of T7 DNA (Molecular Size = 40 Kbp) was studied in a flow device that generates converging flow rather than simple shear flow. We discovered that the sizes of the degradation products were very broadly distributed, covering the range from 10 Kbp to 36 Kbp. An explanation for the broadness of the distribution is given based on a computer simulation of the experiment. The significance of converging flow to the routine handling of large DNA is emphasized.

MeSH Terms

Computer Simulation DNA, Viral Electrophoresis, Agar Gel T-Phages/genetics

Chemicals

DNA, Viral

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Zimm B H

Department of Chemistry, University of California, San Diego, La Jolla 92093.

Reese H R

References (3)

3 references, click to expand

Shear degradation of DNA.
Nucleic Acids Res. 1977;4(5):1513-37 PMID: 19729
Hydrodynamic shear breakage of DNA.
Biopolymers. 1972;11(12):2601-24 PMID: 4649736
THE EFFECT OF HYDRODYNAMIC SHEAR ON THE DEOXYRIBONUCLEIC ACID FROM T(2) AND T(4) BACTERIOPHAGES.
Proc Natl Acad Sci U S A. 1959 Nov;45(11):1560-8 PMID: 16590544

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/18.15.4469

Published

1990-08-11

Pages

4469-70

Language

English

Region

England

NLM ID

0411011

PMCID

PMC331266

Subset

External Links

PubMed source DOI: 10.1093/nar/18.15.4469 PMC Full Text View journal details

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The degradation of T7 DNA in converging flow.

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